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We'll soon get to see the brand's first EV; first, a more honed V12 four-seater
This study aimed to determine the effectiveness of parentage verification in Arabian and Thoroughbred horses in Türkiye using microsatellite markers. A total of 813 Arabian and 959 Thoroughbred horses were genotyped using a total of 17 microsatellite markers. The mean effective number of alleles was 3,34 and the mean number of alleles was 7,41 in Arabian horses. It was calculated that the mean He and Ho values in Arabian horses were 0,677 and 0,680, respectively. The mean effective number of alleles was 3,55 and the mean number of alleles was 6,59 in Thoroughbred horses. It was calculated that the mean Ho and He values in Thoroughbred horses were 0,697 and 0,684, respectively. When the studied Arabian and Thoroughbred horse populations are considered as a single population, the mean FIT, FST and FIS values were found to be 0,063, 0,074 and, - 0,011, respectively. Also, 4 loci in Arabian horses and 3 loci in Thoroughbred horses significantly deviated from HWE. The mean PIC value was 0,63 in Arabian horses and 0,64 in Thoroughbred horses. As a result; the microsatellites including the most informative 15 and 9 loci had a total value of > 0,9999 (11 and 7 loci > 0,999) in each population for PE - 1 and PE - 3, respectively. It has been concluded that parentage verification and genetic identification can be made successfully in the Arabian and Thoroughbred horse populations by using the microsatellite markers panel. Ziel dieser Studie war es, die Abstammungsüberprüfung bei Araber- und Vollblutpferden in der Türkei mithilfe von Mikrosatellitenmarkern zu evaluieren. Insgesamt wurden 813 Araber- und 959 Vollblutpferde mit insgesamt 17 Mikrosatellitenmarkern genotypisiert. Bei Arabern betrug die durchschnittliche effektive Anzahl an Allelen 3,34 und die durchschnittliche Anzahl an Allelen 7,41. Die mittleren He- und Ho-Werte betrugen 0,677 bzw. 0,680. Bei Voll­blütern betrug die durchschnittliche effektive Anzahl an Allelen 3,55 und die durchschnittliche Anzahl an Allelen 6,59. Die mittleren Ho- und He-Werte betrugen 0,697 bzw. 0,684. Betrachtet man die untersuchten Araber- und Vollblutpferdepopulationen als eine einzige Population, betrugen die mittleren FIT-, FST- und FIS-Werte jeweils 0,063, 0,074 und – 0,011. Ausserdem wichen 4 Loci bei Araberpferden und 3 Loci bei Vollblutpferden deutlich vom HWE ab. Der mittlere PIC-Wert betrug 0,63 bei Araberpferden und 0,64 bei Vollblutpferden. Die Mikrosatelliten einschließlich der informativsten 15 und 9 Loci hatten in jeder Population einen Gesamtwert von > 0,9999 (11 und 7 Loci > 0,999) für PE – 1 bzw. PE – 3. Die Überprüfung der Abstammung und die genetische Identifizierung bei Araber- und Vollblutpferdepopulationen können mithilfe des Mikrosatelliten-Markerpanels erfolgreich durchgeführt werden. Cette étude visait à déterminer l’efficacité de la vérification de la parenté chez les chevaux arabes et pur-sang de Turquie à l’aide de marqueurs microsatellites. Au total, 813 chevaux arabes et 959 chevaux pur-sang ont été génotypés à l’aide de 17 marqueurs microsatellites. Le nombre effectif moyen d’allèles était de 3,34 et le nombre moyen d’allèles était de 7,41 chez les chevaux arabes. Il a été calculé que les valeurs He et Ho moyennes chez les chevaux arabes étaient respectivement de 0,677 et 0,680. Le nombre effectif moyen d’allèles était de 3,55 et le nombre moyen d’allèles était de 6,59 chez les chevaux pur-sang. On a calculé que les valeurs moyennes de Ho et de He chez les chevaux pur-sang étaient respectivement de 0,697 et de 0,684. Lorsque les populations étudiées de chevaux arabes et de chevaux pur-sang sont considérées comme une seule population, les valeurs moyennes de FIT, FST et FIS sont respectivement de 0,063, 0,074 et – 0,011. En outre, 4 loci chez les chevaux arabes et 3 loci chez les chevaux pur-sang s’écartent de manière significative du HWE. La valeur PIC moyenne était de 0,63 chez les chevaux arabes et de 0,64 chez les chevaux pur-sang. Par conséquent, les microsatellites comprenant les 15 et 9 loci les plus informatifs avaient une valeur totale > 0,9999 (11 et 7 loci > 0,999) dans chaque population pour l’EP – 1 et l’EP – 3, respectivement. Il a été conclu que la vérification de la parenté et l’identification génétique peuvent être effectuées avec succès dans les populations de chevaux arabes et de pur-sang en utilisant le panel de marqueurs microsatellites. Questo studio aveva l’obiettivo di determinare l’efficacia della verifica dell’ascendenza nei cavalli Arabi e Purosangue in Turchia utilizzando marcatori microsatellitari. Un totale di 813 cavalli Arabi e 959 cavalli Purosangue sono stati genotipati utilizzando un totale di 17 marcatori microsatellitari. Il numero effettivo medio di alleli era di 3,34 e il numero medio di alleli nei cavalli Arabi era di 7,41. È stato calcolato che i valori medi di He e Ho nei cavalli Arabi fossero rispettivamente 0,677 e 0,680. Il numero medio effettivo di alleli era di 3,55 e il numero medio di alleli era di 6,59 nei cavalli Purosangue. È stato calcolato che i valori medi di Ho e He nei cavalli Purosangue fossero rispettivamente 0,697 e 0,684. Quando le popolazioni di cavalli Arabi e Purosangue studiate sono considerate come una singola popolazione, i valori medi di FIT, FST e FIS erano rispettivamente di 0,063, 0,074 e -0,011. Inoltre, 4 loci nei cavalli Arabi e 3 loci nei cavalli Purosangue deviavano significativamente dall’equilibrio di Hardy-Weinberg. Il valore medio di PIC era 0,63 nei cavalli Arabi e 0,64 nei cavalli Purosangue. In conclusione, i microsatelliti, compresi i 15 e i 9 loci più informativi, avevano un valore totale di > 0,9999 (11 loci e 7 loci > 0,999) in ciascuna popolazione per rispettivamente PE – 1 e PE – 3. È stato quindi concluso che la verifica dell’ascendenza e l’identificazione genetica possono essere effettuate con successo nelle popolazioni di cavalli Arabi e Purosangue utilizzando il pannello di marcatori microsatellitari.
Purosangue Orientale Siciliano, Sanfratellano and Siciliano represent the Sicilian equine genetic resource. This study aimed to investigate the genetic diversity, population structure and the pattern of autozygosity of Sicilian horse populations using genome-wide single-nucleotide polymorphism (SNP) data generated with the Illumina Equine SNP70 array. The genotyping data of 17 European and Middle East populations were also included in the study. The patterns of genetic differentiation, model-based clustering and Neighbour-Net showed the expected positioning of Sicilian populations within the wide analysed framework and the close connections between the Purosangue Orientale Siciliano and the Arab as well as between Sanfratellano, Siciliano and Maremmano. The highest expected heterozygosity (He ) and contemporary effective population size (cNe) were reported in Siciliano (He  = 0.323, cNe = 397), and the lowest were reported in Purosangue Orientale Siciliano (He  = 0.277, cNe = 10). The analysis of the runs of homozygosity and the relative derived inbreeding revealed high internal homogeneity in Purosangue Orientale Siciliano and Arab horses, intermediate values in Maremmano and Sanfratellano and high heterogeneity in the Siciliano population. The genome-wide SNP analysis showed the selective pressure on Purosangue Orientale Siciliano towards traits related to endurance performance. Our results underline the importance of planning adequate conservation and exploitation programmes to reduce the level of inbreeding and, therefore, the loss of genetic diversity.
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