Refined vegetable oils depend on tocopherols as their primary natural antioxidants, yet their behavior differs from that observed in model-stripped systems. This study evaluated how tocopherol homologue degradation relates to the lipid oxidation lag phase in refined soybean oil (RSO) and stripped soybean oil (SSO). Tocopherol depletion, lipid hydroperoxides, and headspace hexanal were monitored during accelerated oxidation at 55 °C. In RSO, α-tocopherol depletion coincided with the end of the hexanal lag phase (12 days), despite 56% and 74% of (γ+β)- and δ-tocopherol remaining, respectively, suggesting limited antioxidant contributions from these homologues. In contrast, in SSO, all tocopherol homologues, whether added individually or in combinations, were depleted by more than 90% by the end of the lag phase, indicating that each homologue actively participated in inhibiting lipid oxidation. These differences are likely related to association colloids in refined oils that restrict homologue activity, highlighting opportunities to improve shelf life by enhancing tocopherol effectiveness.
Interpretability is highly desirable for oncologic outcome prediction, as it increases the level of transparency and trustworthiness of the model. This model characteristic is particularly relevant in the setting of modest sample size. Existing work has focused on Shapley Additive Explanations to provide post-hoc explanations on black-box models. These models are not intrinsically interpretable. In this study, we investigated the applicability of an intrinsically interpretable glass-box model, Explainable Boosting Machine (EBM), for hypothesis generation from an early-stage lung cancer data set. We applied EBM to a stripped data set on postradiation therapy lung cancer recurrence, aiming to extract as much information as possible by using pristine EBM configurations. We compared the key features ranked by EBM with those identified through univariate statistical analysis. Additionally, we benchmarked its performance against logistic regression and random forest models, while also evaluating the hypotheses generated by EBM. This study was approved by an institutional review board at the University of Pennsylvania. EBM identified primary tumor size and body mass index as the most prognostic features, aligning with the results of the univariate analysis. Its interpretability provides safeguards against misinterpretation; the model revealed potential age-related bias in this single-arm data set and possible confounding interactions between race and body mass index. EBM yielded competitive performances and more interpretable insights compared with logistic regression and random forest but was not immune from generalizability challenges arising from limited data. The modest performance prevents EBM from being used as a clinical decision support tool, when applied to limited data. However, its interpretable, glass-box nature makes it useful for hypothesis generation.
Denitrifying phosphorus removal (DPR) systems are favored for their ability to conserve carbon sources, yet the widespread practice of iron dosing to stabilize these systems often overlooks the biological threshold of the resident denitrifying phosphorus-accumulating organisms (DPAOs). This study deciphered the multi-tiered collapse mechanism of a DPR system under chronic Fe(III) stress (0-25 mg/L) over 120 days. Macroscopically, a distinct tipping point was identified at 15 mg/L, and exceeding this threshold caused total phosphorus and COD removal to decrease to 45.2 ± 1.9 % and 58.6 ± 2.2 %, respectively. This functional failure was triggered by a cascade of microscopic disintegrations. Structurally, excessive iron stripped the extracellular polymeric substance (EPS) barrier, with a transition in protein secondary structure from ordered states (α-helix) to disordered conformations (β-sheet/random coil). Metabolically, this physical damage forced a critical resource reallocation: intracellular carbon flux was diverted from anabolic polyhydroxyalkanoate synthesis toward energy-intensive antioxidant defenses to counter a 3.5 ± 0.1-fold increase in the production of reactive oxygen species. Genetically, this survival mode was underpinned by the downregulation of quorum sensing and two-component system genes, effectively silencing the chemical communication required for biofilm coordination. Consequently, the abundance of the keystone genus Candidatus Accumulibacter collapsed by 40.0 ± 1.8 %, giving way to stress-tolerant Pseudomonas. These findings confirm that iron overload dismantles DPR systems not just through toxicity, but by structurally uncoupling the metabolic and genetic networks essential for nutrient removal, highlighting the urgent need for bio-centric dosing controls.
The belief in the power of the imagination of parents, and especially of the mother, to influence the features of the foetus has been widespread since ancient times. The first reference is in Genesis 30:25-43. It tells the story of how Jacob showed his father-in-law's ewes and goats coloured branches (stripped branches) during the mating season in order to produce spotted offspring. The theory refers not only to animals, but also to human beings, as various sources testify. The power of female imagination could extend from the moment of coitus through to the entire pregnancy, giving rise to congenital features in the foetus. Starting from the widespread idea of the female imagination as a disruptive factor in the entire process of the formation of the future individual, the aim of this article is to focus on one specific aspect of the question: namely, the origin and development of the belief that 'bastard' children resemble their putative fathers rather than their real fathers. To this end, the most significant medical and philosophical sources will be considered, from the origin of this belief, in the late Middle Ages through to the early modern period.
Surfactants and nanoparticles play a crucial role in regulating the properties of the oil-water interface. However, their influence on the initiation and migration patterns of residual oil in pores remains insufficiently characterized. In this study, nanocompounded systems were prepared by dispersing hydrophobic silica nanoparticles (SiO2-N) in solutions of linear and branched alkylbenzenesulfonate betaines (ASB and XSB), respectively. The effects of these compounded systems on the oil/water/solid interface properties were determined through interfacial tension measurements, emulsion observations, and oil-film stripping tests. The results showed that the addition of SiO2-N enabled the ASB- and XSB-compounded systems to exhibit low interfacial tension characteristics and strong oil film stripping capabilities. Meanwhile, the linear ASB-compounded system demonstrated stronger emulsion stability and further reduced the water separation rate of the emulsion. Additionally, in microscopic-scale oil displacement experiments, the oil displacement efficiencies of the ASB- and XSB-compounded systems increased by 14.9% and 6.5%, respectively. The analysis of remaining oil images indicated that the nanocompounded systems better stripped the aggregated columnar remaining oil, increasing the proportion of liquid and droplet remaining oil. That is to say, the ultralow interfacial tension of the nanocompounded system at the oil/water interface prompts the remaining oil in the pores to transform from columnar to film-like and droplet-like; the improvement in emulsion stability enabled the emulsified oil droplets to have better migration capabilities; the enhanced oil film stripping improved the initiation and migration capabilities of remaining oil, thereby increasing the oil displacement efficiency. Therefore, this paper investigates the initiation and migration characteristics of crude oil in porous media by changing the hydrophobic groups of zwitterionic surfactants of both genders and reveals the synergistic oil displacement mechanism of the compounded system of straight-chain and branched zwitterionic surfactants and nano-SiO2 particles.
Academic nursing does not simply have a diversity problem; it has a knowledge problem. The discipline's credentialing systems, editorial norms, accreditation frameworks, and theoretical canon function as gatekeeping mechanisms that shape whose scholarship is recognized as legitimate, whose methods are considered rigorous, and whose expertise is institutionally validated. Although critical theoretical frameworks have increasingly entered nursing discourse, their structural implications are frequently diluted or neutralized within institutional practice. The purpose of this paper is to examine academic nursing as an epistemological gatekeeper and to analyze how nursing's knowledge infrastructure sustains racial hierarchy while simultaneously performing commitment to equity. Drawing on Critical Race Theory (CRT), and specifically Cheryl Harris's concept of whiteness as property, this conceptual analysis examines the structural and epistemic mechanisms through which academic nursing regulates legitimacy, knowledge production, and disciplinary authority. The paper also considers common diversity, equity, and inclusion (DEI) counterarguments within nursing institutions. The analysis argues that epistemic gatekeeping within academic nursing is neither accidental nor benign. Critical frameworks such as CRT are often incorporated into nursing discourse only after being stripped of structural force and rendered institutionally safe. These dynamics influence who teaches nursing, what nurses are taught, and the profession's capacity to fulfill its stated commitments to health equity. The paper identifies mechanisms of epistemic exclusion and examines how institutional norms reproduce racialized hierarchies of knowledge and authority. Academic nursing must confront not only issues of representation, but also the epistemological structures that govern legitimacy and authority within the discipline. Structural reform is necessary to address entrenched forms of epistemic gatekeeping and to advance meaningful institutional accountability in nursing education and scholarship.
Carbon-based materials are steadily emerging as a potential solution to reduce the use of metals in hydrogen generation. The role of the amount of different oxygen-based functional groups in the ammonia borane hydrolytic decomposition to hydrogen was investigated by functionalising with a strong oxidising agent, HNO3, carbon materials with different graphitisation degrees, i.e., pyrolitically stripped carbon nanofibers (CNFs), graphite and graphene nanoplates (GNP). Results obtained with XPS and Raman spectroscopy studies indicate that the oxygen content increases due to an increase in the quantity of defects in the original materials: O-GNP < O-Graphite ≪ O-CNFs. In order to further refine the results in terms of the nature of the oxygen functionalities introduced, a mild oxidising agent was also employed on CNFs, i.e. hydrogen peroxide (H2O2). Indeed, carbonyl and carboxyl groups are predominant under strong oxidizing conditions, while ether-like and hydroxyl groups were introduced using H2O2. The presence of CO bonds has a positive effect on hydrogen production, resulting in an increase of approximately one order of magnitude from O-Graphene to O-CNFs. Quantum chemical density functional theory calculations were used to obtain some atomistic insight into the activation of ammonia borane and to assess the effect of oxygen functionality. The results of this study provide evidence of the positive effects induced by the presence of C-O double bonds, suggesting a viable method for further catalytic optimization toward the generation of hydrogen from ammonia borane.
The Amsterdam University Medical Centers IMAging in GliOma (IMAGO) dataset contains 1,700 adult-type diffuse glioma patients with structural MRI, histological, molecular, and survival information. Its first release includes 500 patients with preoperative structural MRI (T1w pre-contrast, T1w post-contrast, T2w, and T2-FLAIR). Postoperative MRI with the same sequences as the preoperative MRI are included for patients scanned within 14 days of surgery (381 patients). Pre- and postoperative apparent diffusion coefficient (ADC; derived from diffusion-weighted MRI; 497/500 and 377/381 respectively) and relative cerebral blood volume (rCBV; derived from dynamic susceptibility contrast MRI; 269/500 and 24/381 respectively) images are provided. All sequences are registered to MNI standard space and skull stripped. Histological and molecular data include IDH (500/500) and 1p/19q codeletion status (170/500), and WHO CNS5 (2021) tumor grade (500/500). Preoperative and postoperative nnUnet-based tumor region segmentations with human quality check are available for a subgroup of cases. The dataset is usable to create synthetic images, tumor segmentations, and to predict histological and molecular features in adult-type diffuse glioma.
Objectives: Titanium plates may offer advantages in fracture healing, yet concerns remain regarding hardware removal due to risks such as screw stripping and cold welding. Whether titanium removal is more difficult than stainless steel remains uncertain. This study compared the ease of implant removal, focusing on distal femur fractures treated with titanium versus stainless-steel plates. We conducted a retrospective cohort study of patients with distal femur fractures who underwent implant removal after fixation with either stainless-steel or titanium plates. The primary outcome was difficulty of removal, including cold welding, screw stripping, hardware breakage, and use of advanced tools (screw removal set, trephine, burr). The secondary outcome was operative duration. Seventy-two patients were included: 31 stainless steel and 41 titanium. Mean in-vivo implant time was 421 ± 498 days for stainless steel and 360 ± 409 days for titanium (P = 0.57). Difficulties with removal occurred in 13% of stainless-steel cases and 12% of titanium cases (P = 0.92). Screw removal sets were required in 9.7% and 9.8% of patients, respectively (P = 0.99). Advanced extraction tools were used in 3 patients per group (P = 0.72). Cold-welded or stripped screws occurred in 2 patients in each group (P = 0.77). Mean operative time was 155 ± 80 minutes for stainless steel versus 118 ± 67 minutes for titanium (P = 0.06). In distal femur fractures, titanium plate removal is not associated with increased technical difficulty compared with stainless steel implants.
Conventional open-pit production scheduling models often neglect environmental costs, government royalties, and operational penalties, resulting in economically optimistic but practically unrealistic mine plans. This study develops a Mixed-Integer Non-Linear Programming (MINLP) model for long-term production scheduling at the Kal-e Kafi copper deposit in Iran. The model explicitly integrates environmental costs, government royalties, and penalty costs for deviations from target mill feed tonnage and grade directly into the objective function and block economic value calculation. Implemented on a scaled block model comprising 20,746 selective mining units and solved using the BARON 23.1.7 solver within a Divide and Conquer framework, the MINLP model achieves a net present value of $129.34 million, a stripping ratio of 0.64:1, a mine life of 14 years, and extractable ore tonnage of 14.24 million tonnes. Comparative analysis against the industry-standard Datamine NPV Scheduler reveals that although the commercial software reports a higher nominal NPV, it does so by externalizing environmental liabilities, royalty obligations, and operational penalties. The MINLP model demonstrates comparatively better operational efficiency through a lower stripping ratio, a longer mine life, and higher ore recovery. A comprehensive sensitivity analysis confirms model robustness, with the environmental cost elasticity near zero, indicating that internalizing environmental expenditures does not compromise economic competitiveness. These findings demonstrate that responsible mining produces field-realistic, operationally feasible, and economically defensible mine plans, bridging the gap between theoretical optimization and practical sustainability.
Background/Objectives: Psoriasis is a chronic immune-mediated skin disease, with plaque psoriasis being its most prevalent form. Although biologic therapies have significantly improved outcomes for patients with moderate to severe disease, certain anatomical regions often remain resistant to treatment. Given the observed variability in treatment response depending on lesion location, we aimed to explore anatomical site-specific differences in the skin transcriptome. Methods: Using a non-invasive tape-stripping technique, we collected and analyzed 44 psoriatic plaque samples from the scalp, trunk, upper extremities, and lower extremities, followed by differential gene expression and gene ontology analysis. Moreover, we included 80 samples obtained from healthy skin biopsies (GSE54456) to conduct an inflammation-controlled approach. We used two different approaches, an intra-disease approach, in which anatomically different sites were compared, and an inflammation-controlled approach, in which the inflammation bias was reduced. Results: Our findings indicate distinct molecular signatures and biological pathways across different anatomical sites, including differential expression of SERPINB7 and miRNAs such as miR-205 and miR-203a, along with different pathways. Furthermore, our results emphasized the heterogeneity of psoriasis and suggested that site-specific molecular mechanisms may contribute to variations in disease manifestation and treatment response. Conclusions: This study highlights the need for more personalized, site-specific therapeutic strategies. It should be considered an exploratory pilot study, and larger studies with paired samples from multiple anatomical sites within the same patients are needed to validate the identified transcriptomic signatures.
Sulfides-based all-solid-state lithium batteries show great potential due to their high safety and high energy density, yet severely suffer from sulfide electrolytes/Li interfacial instability and short cycle life. Here we propose a Li-In-S composite foil comprising Li2S, LixIn, and LiInS2 to stabilize the Li/Li6PS5Cl interface by constructing built-in electric fields, thereby enabling long-life all-solid-state lithium batteries. The work function difference between Li2S and LixIn generates built-in electric fields at the heterointerface, which traps the interfacial electrons and restricts their transfer to Li6PS5Cl, thereby suppressing the interfacial side reactions. Simultaneously, the built-in electric fields promote Li+ adsorption and diffusion inhibiting lithium dendrite growth. Li symmetrical cells display high critical current density over 4 mA cm-2 and Li plating/stripping stability over 2000 h at 1 mA cm-2. The assembled full cells with LiCoO2 and LiNi0.8Co0.1Mn0.1 (NCM811) demonstrate high capacity retention of 93% over 2000 cycles at 1 C and 87.7% over 1000 cycles at 1 C, respectively. Moreover, Li-In-S|Li6PS5Cl|NCM811 full cell shows rate capability up to 4 C. This work offers useful insights into the design of stable interfaces in all-solid-state batteries.
Bullous keratopathy may lead to severe corneal opacity and impaired visualization of anterior segment structures, complicating surgical qualification for endothelial keratoplasty (EK). We report the case of a 67-year-old male with pseudophakic bullous keratopathy and Fuchs endothelial dystrophy presenting with clinically complete corneal opacity and visual acuity limited to hand motion. Slit-lamp examination and anterior segment optical coherence tomography demonstrated marked epithelial remodeling with a dense plaque-like surface lesion obscuring deeper corneal structures. A staged intraoperative approach was undertaken. Following mechanical epithelial debridement, partial restoration of corneal transparency allowed for an intraoperative reassessment of stromal clarity and subsequent Descemet Stripping Automated Endothelial Keratoplasty (DSAEK). Histopathological examination demonstrated reactive epithelial thickening with associated subepithelial fibrosis consistent with chronic bullous keratopathy. Postoperatively, corneal transparency was restored and best-corrected visual acuity improved to 0.7 Snellen (0.15 logMAR), remaining stable during follow-up without graft-related complications or recurrent epithelial abnormalities. This case highlights the importance of considering epithelial contributions to apparent corneal opacity in advanced bullous keratopathy and suggests that staged intraoperative reassessment may support individualized surgical decision-making in selected patients with inconclusive preoperative evaluation.
Anode-less aqueous zinc batteries offer a promising route to energy-dense and intrinsically safe energy storage, yet their practical deployment is hindered by poor reversibility under constrained zinc inventory. Here, we identify galvanic corrosion between deposited zinc and current collectors as a critical but previously underappreciated degradation pathway in anode-less aqueous zinc batteries. A hybrid passivation layer, comprising an electrically insulating polymer matrix embedded with high-permittivity metal fluorides, is designed to mitigate parasitic electron transfer and limit current collector exposure. The resulting dielectric polarization homogenizes the interfacial electric field, promoting planar and dense zinc deposition that isolates the current collector and suppresses corrosion. Consequently, irreversible zinc loss during both cycling and calendar ageing is reduced, enabling stable zinc plating/stripping for over 900 h at 5 mA cm-2/3 mAh cm-2 with 51% depth of discharge. Ampere-hour-scale anode-less full cells deliver 89% capacity retention after 130 cycles at 0.5 A g-1, with minimal self-discharge during calendar ageing, and initially anode-free pouch cells achieve device-level energy densities exceeding 90 Wh L-1. This work advances the mechanistic understanding of corrosion processes and motivates corrosion-conscious engineering of heterogeneous interfaces across broader metal battery systems.
Lithium metal batteries (LMBs) employing LiNi0.5Mn1.5O4 (LNMO) cathodes (5 V-class, vs Li+/Li) exhibit significant potential for next-generation energy storage owing to their high theoretical energy density and high-voltage capability. However, the practical development of LNMO/Li batteries is severely constrained by the incompatibility of carbonate-based electrolytes with highly reactive cathodes and anodes. To solve this, we propose a novel electrode-electrolyte interfacial engineering strategy: a dry electrode fabrication process is implemented, in which vapor-grown carbon fibers (VGCFs) are coated with polytetrafluoroethylene (PTFE) to passivate carbon active sites, thereby effectively mitigating electrolyte oxidation at high voltages. Simultaneously, lithium nonafluorobutanesulfonate (LNBS) and succinonitrile (SN) are incorporated as functional additives into the cathode. Mild heating during electrode fabrication melts these additives, enhancing the flexibility and mechanical integrity of the electrode. These additives partially dissolve during cell operation. LNBS contains SO3- polar groups that facilitate the dissociation of lithium salt ion pairs. Its electronegativity anchors transition metal ions onto the LNMO cathode surface, forming a Ni-S-containing adsorption layer that suppresses their migration into the electrolyte. Meanwhile, lithium difluoro(oxalato)borate (LiODFB) preferentially decomposes at the cathode interface, cooperatively forming a protective bilayer (an outer inorganic-rich layer and an inner Ni-S-containing absorption layer). At the anode, Li-SN (formed via coordination between SN and Li+) decomposes to generate an inorganic-rich solid electrolyte interphase (SEI), stabilizing lithium plating/stripping. These synergistic modifications enable LNMO/Li batteries with high-loading cathodes (20 mg cm-2) to achieve 88.6% capacity retention after 400 cycles at 0.5 C, with an average Coulombic efficiency of 99.44%. Moreover, when charged to 4.85 V, the cell maintains an open-circuit voltage above 4.68 V for over 1400 h, demonstrating exceptional cycling and storage stability with conventional carbonate-based electrolytes.
Bisphenol E (BPE) and bisphenol B (BPB) are emerging as alternatives to bisphenol A (BPA), although information on their toxicity and human exposure remains limited. While not formally classified as such, both compounds are suspected endocrine disruptors. To better evaluate potential occupational exposure, this study investigated their dermal absorption, following OECD guidelines for in vitro experiments. Experiments were conducted using freshly-excised human skin mounted on Franz diffusion cells. Skin samples were exposed for 20 h to radiolabelled BPE or BPB. Time-course analyses were used to determine key toxicokinetic parameters, including steady-state flux, lag time, and skin permeability coefficient (Kp). After exposure, compound distribution and potential skin reservoir effects were assessed by sequential tape-stripping and separation of epidermis and dermis. Permeability coefficients were determined: 4.2 × 10-3 cm/h for BPE; 3.4 × 10-3 cm/h for BPB. Approximately 49% of the applied dose of BPE and 36% of the applied dose of BPB were absorbed, whereas 20% and 29%, respectively, were retained in the skin. Overall, the results demonstrate substantial dermal absorption of BPE and BPB and highlight skin retention as a factor contributing to potential prolonged systemic exposure. These data provide important information for regulatory risk assessment.
Fe-based amorphous alloys have attracted increasing attention as potential current-collector materials because of their unique atomic structure and corrosion resistance. In this work, Fe78Si13B9 amorphous-alloy ribbons were employed as current collectors to investigate the influence of annealing treatment on the electrochemical behavior of Li||current collector half-cells. DSC and XRD analyses indicated that partial crystallization occurred after annealing at 480°C, while the degree of crystallization further increased after annealing at 580°C. SEM observations revealed obvious changes in surface morphology and crystallization features after annealing treatment. Electrochemical measurements showed that the Fe78Si13B9 alloy annealed at 580°C exhibited relatively improved cycling stability and smaller polarization evolution compared with untreated alloy and Cu foil under the present testing conditions. The sample maintained a Coulombic efficiency (CE) of approximately 97% after 150 cycles at 1.0 mA cm-2. In addition, EIS and SEM observations of Li deposition morphology suggested relatively lower interfacial impedance and comparatively denser Li deposition morphology after annealing treatment. This work provides insight into the relationship between annealing-induced structural evolution and Li deposition behavior of Fe-based amorphous-alloy current collectors.
The practical application of Li metal anodes is hindered by uncontrollable dendrite growth, which stems from the unstable solid electrolyte interphase (SEI) and heterogeneous Li+ flux. Herein, we report a porous Bi4Ti3O12 (BTO) decorated with in-situ generated titanium oxide and bismuth nanocrystals (denoted as BTO-TiOx/Bi-NC) composite, as a multifunctional interfacial layer to address these challenges. The integrated TiOx ensures high ionic conductivity, while Bi-NC effectively regulate Li+ flux, thereby guiding uniform Li+ plating/stripping. Moreover, it is hypothesized that the reversible Li3Bi alloying reaction during cycling could provide additional nucleation sites, potentially contributing to enhanced Li deposition homogeneity. Benefiting from this rational design, BTO-TiOx/Bi-NC promises the symmetric Li||Li cell exhibiting remarkable stability for 800 h at 1 mA cm-2 in high-voltage ester-based electrolyte. When coupled with an S cathode, the full LiS battery maintains a discharge capacity of 466.53 mAh g-1 after 400 cycles at 3C in ether-based electrolyte, demonstrating excellent long-term cyclability. This work proposes a promising strategy for stabilizing Li metal anodes via combined Li+ flux regulation and alloying chemistry, paving the way for durable high-energy-density batteries.
The electrochemical ammonia stripping (EAS) exhibits considerable potential for the removal and recovery of ammonia nitrogen from biogas slurry. However, its practical application is constrained by the requirement for sulfuric acid as an absorbent, resulting in the formation of acidic nitrogen fertilizer (NF) that lacks suitability for direct application. A combined process integrating EAS with calcium-based ammonia absorption was proposed to address the above limitation. Both solid and liquid phases were characterized throughout the reaction, providing insight into the reaction progression. Based on the results of single-factor optimization, response surface methodology was employed to further optimize the CO2 concentration, aeration flow rate, current intensity, and temperature, achieving a total ammonia recovery efficiency of 96.1 % and a CaSO4 conversion rate of 20.4 %. Notably, the resulting NF exhibited a pH in the range of 6.5-8.5, making it suitable for direct agricultural use. Furthermore, a germination index of 109 % was achieved with the product diluted to 4 %, confirming the NF is no acute phytotoxicity under the tested conditions. In pot experiments with Chinese cabbage, substituting 75 % of urea with the NF resulted in a dry weight of 2.9 g, representing a 20.9 % increase compared to the non-substitution treatment, and the nitrogen use efficiency improved from 5.1 % to 10.2 %. This study provides a cost-effective and sustainable approach for NF production, contributing to the circular utilization of waste resources.
Skin tape stripping is a minimally invasive, skin-specific method for biomarker collection that has gained increasing interest in dermatologic research. However, the lack of standardized protocols for protein and nucleic acid extraction from tape strips has limited its broader application. This study aimed to establish robust and broadly compatible protocols for biomarker extraction from tape strip samples to enable reliable downstream proteomic and transcriptomic analyses. We systematically evaluated key technical parameters influencing biomarker recovery, including collection and storage conditions, buffer composition, and lysis strategies. Protein and nucleic acid extraction protocols were optimized for maximum yield and quality across a range of downstream applications, including immunoassays and transcriptomic profiling. The optimized protein extraction protocol demonstrated improved total yield and compatibility with multiple assay platforms. A parallel nucleic acid extraction method yielded high-quality RNA suitable for gene expression analyses. Notably, we found that disease-relevant biomarkers were detectable in the most superficial tape strips, indicating that fewer total strips may be sufficient for effective analysis. We present practical and standardized methods for protein and nucleic acid extraction from skin tape strip samples. These protocols address current technical barriers and support broader implementation of tape stripping as a biomarker collection strategy in clinical dermatology and related fields.