Animals feature regularly on children's television, exposing children to implicit and explicit messages about the natural world. Although animal representation and anthropomorphism have been examined across children's media, film, literature, and popular culture, less is known about how animals are portrayed in preschool television, particularly in relation to biological and ecological accuracy. This study examined animal representations in programs aired on ABC Kids, the Australian Broadcasting Corporation's free-to-air children's television channel, using quantitative and qualitative content analysis. A total of 947 episodes from 92 programs were analyzed, with a subset of 265 episodes (43 h and 45 min) from 39 programs examined in greater detail. Animals were prominently featured, appearing on screen for 88% (171 h and 40 min) of total programming time, with programs featuring animals as central characters accounting for 41% (81 h). Nearly all central animal characters exhibited anthropomorphic traits, such as walking, talking, dressing, or behaving like humans. Inaccuracies in species depiction were common, including portrayals of animals in human-like social structures, unrealistic environments, and mixed predator-prey groupings, alongside a hierarchy based on roles and relationships. These findings indicate that realistic portrayals of animals and the natural world were uncommon. The study highlights the need for greater consideration of how animal characters are represented in preschool television, and how imaginative animal portrayals might be balanced with biologically and ecologically grounded depictions. Further research should examine how such portrayals influence children's understanding of animals and their connection with nature.
Ammonia exposure can induce oxidative stress in aquatic animals. The p62 protein is a selective autophagy receptor that participates in protein degradation and oxidative stress regulation. In this study, the role of Lv-p62 in the response of Litopenaeus vannamei to ammonia exposure was investigated using RNA interference. The results showed that Lv-p62 expression was significantly induced in the hepatopancreas, gills, and intestine of L. vannamei after ammonia exposure (p < 0.05). Lv-p62 expression peaked at 6 h in the gills and 24 h in the intestine, whereas a biphasic response was observed in the hepatopancreas, with an initial peak at 12 h and a higher second peak at 48 h. In the RNAi experiment, Lv-p62 knockdown altered the expression of antioxidant-related genes (Trx, Gst, and Gpx) in a tissue-specific manner, with Gpx expression being prominently increased in the gills and intestine but not in the hepatopancreas under ammonia exposure. Autophagy-related genes (ATG4 and ATG10) also showed time-dependent and tissue-specific expression changes after Lv-p62 knockdown. The expression of apoptosis-related genes, including caspase 3 and p53, was tissue-specific and was generally lower in the dsRNA-Lv-p62+NH3 group than in the dsRNA-EGFP+NH3 group at most time points. Histopathological observations showed that hepatopancreatic acinar vacuolation and structural damage were alleviated, and the hepatopancreatic apoptosis rate was reduced in L. vannamei in the dsRNA-Lv-p62+NH3 group. These findings suggest that Lv-p62 participates in the response of L. vannamei to ammonia exposure, possibly by regulating antioxidant defense, autophagy-related processes, and apoptosis, thereby affecting hepatopancreatic oxidative damage and tissue injury.
The aim of this study was to identify the main causes of neonatal mortality in dairy calves (0-30 days of age) in Poland and to assess how infectious factors and management practices influenced the pattern of mortality. A retrospective analysis was performed on 498 calves from 312 herds between 2018 and 2024. The protocol included necropsy, enteropathogen diagnostics (PCR and ELISA), and analysis of herd management questionnaires. The leading causes of death were chronic diarrhea (42.4%), acute diarrhea (25.3%), and septicemia (10.8%). Calves that died from septicemia were the youngest (mean age 7.7 days), whereas those that died from chronic diarrhea were the oldest (20.3 days; p < 0.001). Lack of dam vaccination was associated with a higher occurrence of acute diarrhea (RR = 2.04) and septicemia (RR = 2.01) within the necropsied calf population. Inappropriate colostrum management was also associated with a higher occurrence of septicemia (RR = 1.99). Despite widespread antimicrobial use (>80%), intensive fluid therapy was used rarely (<7% in diarrheal cases). ETEC, rotavirus, and coronavirus were detected significantly more frequently in acute diarrhea, whereas Cryptosporidium parvum (C. parvum) was significantly more frequent in chronic diarrhea. Antimicrobials were used extensively, while intensive fluid therapy for diarrhea was implemented infrequently and inadequately relative to disease severity. This study makes an important contribution to the understanding of calf mortality in dairy herds by combining comprehensive postmortem diagnostics with pathogen detection (PCR and ELISA) and the assessment of management practices, thereby providing a holistic perspective on the problem. The analysis of 498 calves from 312 farms over a 7-year period offers an unprecedented and representative picture of the situation in Poland, one of Europe's major milk producers. These results emphasize the crucial importance of proper colostrum management and vaccination, while also indicating a clear and urgent need to expand the use of fluid therapy in calves requiring treatment. The results identify not only the dominant pathogens and differences in disease course, but also management factors with direct implications for animal welfare and preventive strategies in dairy production.
Raptors are high-trophic-level predators and scavengers that are sensitive to habitat alteration, human disturbance, and climate variability, yet province-wide assessments of their habitat suitability and climate-change responses remain limited in subtropical China. Hunan Province, located along the inland section of the East Asian-Australasian Flyway, contains complex mountain systems, plains, wetlands, and land-use mosaics that may support diverse raptor assemblages. Based on raptor survey records collected across Hunan from January 2022 to July 2023, we used biomod2 ensemble species distribution models to assess current habitat suitability, identify key environmental predictors, and project future changes under the SSP2-4.5 and SSP5-8.5 scenarios for the 2050s and 2090s. We recorded 39 raptor species and retained 3637 valid geographic locations and 4855 observed individuals after data cleaning. Nine representative species were further selected to construct 22 species-season combinations covering resident species, summer visitors, winter visitors, and four phenological stages. The EMwmean weighted ensemble model consistently outperformed the best single models, increasing mean AUC from 0.882 to 0.970 and the mean TSS from 0.611 to 0.845. Temperature seasonality (BIO4), the Human Footprint Index (HFP), precipitation in the driest month (BIO14), and the Normalized Difference Vegetation Index (NDVI) were the dominant predictors, although their relative importance varied among residency types and phenological stages. Under current conditions, highly suitable and most suitable habitats covered 65,259.67 km2, accounting for 30.81% of Hunan Province, and were mainly concentrated in western, southern, and eastern mountain regions. Future projections indicated a marked contraction of high-suitability habitats, especially under SSP5-8.5, with no HSI > 0.6 habitat identified by the 2090s. High-suitability habitats also became increasingly concentrated at higher elevations. These findings identify mountain regions as key conservation priorities and provide a spatial framework for climate-adaptive raptor conservation in Hunan Province.
The Ili River Valley and adjacent Xinjiang regions contain introduced cattle, local cattle, and yak and therefore provide a useful regional system for examining cattle-yak genomic differentiation. Using whole-genome resequencing data from 79 individuals, we analyzed Angus (ANG), Simmental (SIM), Holstein (HOL), Xinjiang Brown Cattle (XH), Kazakh Cattle (KAZ), Altay White-headed Cattle (AWH), and yak (WY). The six domestic cattle groups were merged into a composite domestic cattle group (PTN, n = 69) and compared with WY (n = 10). Sequencing generated 2996.28 Gb of raw data and 2939.56 Gb of clean data. Alignment to the Bos taurus ARS-UCD1.2 reference genome yielded mapping rates of 96.71-99.78% and depths of 5.98×-17.26×. Genome-wide PTN-WY comparisons showed extremely high differentiation: the median weighted F_ST was 0.846 and the 95th percentile was 0.943. The joint F_ST-π scan identified 832 candidate highly differentiated windows and 533 unique ENSBTAG gene IDs, whereas the low-differentiation set contained only five windows and three genes. The longest contiguous highly differentiated segments were located on chromosomes 26, 29, 8, 21, and 7. WY had the highest median Tajima's D (1.173) and the slowest LD decay, while KAZ had the lowest median Tajima's D (0.345) and the fastest LD decay. Treemix supported non-tree-like covariance components, and PSMC indicated broadly similar deep-time demographic profiles across individuals. Overall, the dominant genomic signal between PTN and WY is deep phylogenetic divergence, with locally enhanced highly differentiated segments superimposed on this background. These segments were enriched for functions and pathways related to reproductive behavior, neuroendocrine regulation, circadian rhythm, and membrane transport, but they are not interpreted here as recent within-species selective sweeps. The results provide a cautious regional framework for conservation and breeding of bovine genetic resources in Xinjiang.
Cryopreservation of stallion semen is associated with oxidative stress (OS), which can impair sperm function and fertility. This study evaluated antioxidant activities in seminal plasma and sperm cytosols and investigated their relationships with selected sperm functional parameters following cryopreservation, with or without antioxidant supplementation. Semen was collected from ten fertile stallions and processed using a split-ejaculate design, including fresh semen and six freezing treatments: HF-20 extender alone; HF-20 supplemented with matcha, spirulina, horseradish, or quercetin; and a commercial extender (INRA Freeze). Total antioxidant capacity (FRAP) and enzymatic activities (superoxide dismutase, SOD; catalase, CAT; and glutathione reductase, GR) were measured in seminal plasma and sperm lysates. Linear regression analyses revealed significant associations between seminal plasma and fresh spermatozoa with respect to SOD and GR activities. In frozen-thawed semen, FRAP and CAT activities differed between samples cryopreserved with and without antioxidant supplementation. Significant correlations were observed among antioxidant activities, sperm kinetics, OS markers, and DNA fragmentation indices. Principal component analysis provided an exploratory overview of multidimensional patterns of covariation among sperm kinetics, redox balance, and nuclear fragmentation, explaining for 73% of the total variance. Overall, the results suggest complex associations between the antioxidant system and sperm quality and indicate that antioxidant supplementation of freezing extenders may modulate the redox status of stallion sperm after thawing.
Raramuri Criollo (RC) cattle from Mexico have special genetic and resilience characteristics which have positioned them as an important alternative for future meat production under arid conditions. Despite the remarkable hardiness of RC cattle, their meat has been anecdotally catalogued as lean, tough and unattractive for consumption. However, abundant information about RC meat quality has not been documented to date. This study evaluated technological characteristics and tenderness of Longissimus lumborum muscle from Raramuri Criollo cattle compared to a commercial Hereford × Angus (H × A) crossbreed during dry ageing. pH, water-holding capacity (WHC), Warner-Bratzler shear force (SF), and instrumental colour were analyzed at 0, 15 and 30 d of ageing. pH values remained within the normal physiological range (5.4-5.5) for high-quality beef in both genetic groups. WHC showed significant differences among days of ageing, but not by racial group (p > 0.05). Additionally, RC beef demonstrated higher chromatic stability than H × A meat during ageing. Meat from H × A animals exhibited faster early tenderization, while RC beef showed a more gradual and sustained reduction in SF, with RC reaching lower SF than meat from crossbred animals by d 30 of ageing. From day 0 to day 15 SF decreased by 9.48 N and 12.16 N for RC and H × A meat, respectively, while from day 16 to day 30 the SF showed decreases of 4.73 and 2.11 N for RC and H × A meat respectively. In conclusion, RC meat is as tender as H × A after 30 d ageing without deterioration in its technological properties. These findings highlight the potential of RC to produce competitive tender meat, supporting valorisation of indigenous cattle genetic resources as a viable strategy for sustainable beef production in arid environments.
The Apennine wolf (Canis lupus italicus) is a distinct subspecies whose ongoing population recovery in Italy has progressively increased the demand for live capture protocols validated for scientific monitoring and conservation management. Despite the widespread use of mechanical and chemical immobilization in European wolf management, no study has to date systematically evaluated the combined use of the Fremont™ humane foot snare with a medetomidine-ketamine-acepromazine (MKA) protocol in this subspecies, nor characterized the associated cardiorespiratory, thermal, and hematobiochemical parameters under operational field conditions. Between June 2010 and July 2017, thirteen free-ranging Apennine wolves were captured in Maiella National Park (central Apennines, Italy) using the Fremont™ snare and immobilized with a standardized MKA protocol; only animals immobilized with this protocol are reported here, as three additional capture events employed different drug combinations. Cardiorespiratory parameters, body temperature, peripheral oxygen saturation, venous blood gas values, and a comprehensive hematological and serum biochemical panel were recorded during immobilization. Mean heart rate was 100 ± 15 bpm, respiratory rate 24 ± 13 breaths/min, body temperature 38.1 ± 1.3 °C, and mean SpO2 88 ± 11% (range: 66-97%; n = 12). No clinically significant hyperthermia requiring active intervention was recorded in the cohort as a whole. Hematological and biochemical values were broadly consistent with published reference ranges for the species, with condition-specific deviations identified in two individuals-one pregnant female and one juvenile presenting signs of transient capture-related myopathy-both of which resolved without clinical sequelae. No capture-related mortality occurred. All thirteen individuals survived the minimum post-capture monitoring period. Preliminary GPS observations in a subset of individuals (n = 3) suggest a transient reduction in movement activity in the immediate post-release period. These findings support the safety and operational feasibility of the combined Fremont™ snare-MKA protocol for the Apennine wolf, and provide baseline physiological and hematobiochemical reference data for Canis lupus italicus relevant to future capture and conservation management programmes.
Folate is a central nutrient in one-carbon metabolism, contributing to nucleotide biosynthesis, methionine cycling, methyl-donor supply, and epigenetic regulation. In animals, the intestine is both a principal site of folate absorption and a key target organ for folate action. This narrative review focuses primarily on livestock, poultry, aquaculture species, ruminants, and animal-source food enrichment, while also using rodent, human, and in vitro studies as mechanistic or translational evidence. We synthesize evidence on folate absorption, transport, and metabolism and evaluate the mechanisms through which folate influences intestinal health. Available evidence suggests that adequate folate supply may support epithelial renewal, tight-junction integrity, mucosal immune balance, antioxidant capacity, gut microbiota stability, short-chain fatty acid production, and epigenetic regulation of intestinal development. These effects have been reported in poultry, pigs, fish, ruminants, rodents, and maternal-offspring models. However, the evidence is uneven across species, and dose-response relationships, folate forms, bioavailability, and species-specific requirements remain major limitations for translating current knowledge into animal production. Future studies should compare folic acid, 5-methyltetrahydrofolate, natural reduced folates, microbiota-derived folate, and folate-producing probiotics; quantify the contribution of microbiota-derived folate to host methyl-donor pools; and develop precision strategies that integrate folate with other one-carbon nutrients, probiotics, and product-enrichment technologies.
The suckling phase is the critical window for rumen functional maturation, yet amino-acid-based interventions tailored to this stage remain scarce. L-citrulline (L-cit) bypasses hepatic first-pass metabolism, is converted to L-arginine peripherally, and resists ruminal microbial degradation, making it a candidate functional additive for early-life ruminants. This study evaluated whether dietary L-cit at 2 g·lamb-1·d-1 would improve rumen development and metabolic function in suckling Hu lambs. Twenty male Hu lambs were randomly assigned to a control (CON) or L-cit group (n = 10/group) and reared for 45 d (3 d adaptation + 42 d treatment). Growth and starter intake were assessed in all lambs; six lambs per group (n = 6) were subsequently slaughtered for rumen morphometry, gas chromatography-flame ionization detection (GC-FID) volatile fatty acid (VFA) quantification, 16S rRNA gene sequencing, and liquid chromatography-mass spectrometry (LC-MS) untargeted metabolomics. L-cit increased average daily starter intake by 25.96% (p = 0.036) and produced a 20.00% numerical but non-significant increase in average daily gain (ADG) (p = 0.203; Cohen's d = 0.58). Rumen weight, volume, and papillary length, width, density, and epithelial thickness were all elevated (p < 0.05), whereas muscular thickness was unaffected (p = 0.162). Total VFA, acetate, propionate (+37.64%, p < 0.001), and butyrate were higher in the L-cit group; the molar proportion of propionate rose from 21.41% to 24.75%, and the acetate-to-propionate ratio declined from 2.90 to 2.44 (p = 0.005). Microbial richness (Chao1, Observed species) increased without altered evenness, and linear discriminant analysis effect size (LEfSe) identified L-cit-driven enrichment of propionate-generating and fiber-degrading genera, including Prevotellaceae_UCG-004, Ruminobacter, and the NK4A214_group. Of 539 differential metabolites (147 of which were annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database), KEGG enrichment highlighted linoleic acid metabolism and purine metabolism as the biologically interpretable targets. Microbiota-metabolite correlations linked L-cit-enriched genera to up-regulated metabolites such as adenine. Dietary L-cit at 2 g·lamb-1·d-1 enhances starter intake, promotes rumen epithelial development, promotes a shift toward enhanced propiogenic fermentation within an acetate-dominant profile, and remodels the microbiota-metabolome axis, supporting its application as a functional additive during the suckling phase of ruminants. Because epithelial barrier integrity, oxidative stress, and inflammatory markers were not directly measured, these findings should be interpreted as morphological and association-based evidence, and further functional validation is required.
Forest conversion from natural forests to secondary forests and plantations has significantly altered wildlife habitats in subtropical regions. However, the drivers of disparities in bird and mammal multidimensional diversity between these forest types remain poorly understood. We analyzed a four-year camera-trapping dataset to compare the taxonomic, functional, and phylogenetic diversity and community structure of birds and mammals. Our results indicated that forest conversion impacts biodiversity differently across taxa. Birds exhibited higher taxonomic and phylogenetic diversity in secondary forests than in plantations. A similar pattern was also evident for birds among different stand types. However, mammals exhibited considerable taxonomic diversity but showed higher phylogenetic diversity and structure in secondary forests. A similar pattern was also evident for mammals among different stand types. Beta diversity revealed significant differences in bird taxonomic composition and mammal phylogenetic composition between secondary and plantation forests. Furthermore, elevation primarily influenced bird taxonomic diversity, phylogenetic diversity and structure in secondary forests, whereas mammal functional diversity, phylogenetic diversity and structure were more sensitive to elevational changes in both secondary and plantation forests. These findings reveal that birds and mammals respond distinctively to forest conversion. We emphasize that management strategies must be group-specific. For birds, we recommend prioritizing the preservation of secondary forests as biodiversity refugia and transforming structurally simplified plantations into complex habitats by retaining legacy trees and native understory vegetation. For mammals, conservation should prioritize landscape-scale connectivity by protecting continuous forest corridors along altitudinal gradients. Practically, this requires restricting further fragmentation of high-altitude habitats and restoring native vegetation in degraded corridors to facilitate dispersal and maintain the phylogenetic integrity of mammal communities.
Sperm cryopreservation is an integral part of gene pool conservation programs for local poultry breeds. It is known that cryostress can cause significant changes in the expression profiles of microRNAs and their target genes-key players in spermatogenesis-in Gallus gallus domesticus. However, the transmission of these changes across generations remains poorly understood. The aim of this study was to evaluate the transgenerational epigenetic effects of rooster sperm cryopreservation on molecular genetics and histological parameters in the gonads of offspring (F1) during the embryonic (10 days) and postnatal (1 day) periods. The analysis included a comprehensive histomorphometric analysis of the gonads and a quantitative assessment of the expression of microRNAs (gga-miR-6701-3p, gga-miR-301a-5p) and their target genes (DMRT1, TGFB2), using qRT-PCR. Histological analysis of the gonads of 10-day-old embryos revealed early morphological abnormalities in the F1 (n = 10) offspring obtained from frozen-thawed semen (experimental group). It was found that day-old F1 chicks (n = 17) obtained from frozen semen had testes with a significantly reduced number of seminiferous tubules (-36%, p < 0.05) with an increased diameter (+22%, p < 0.05) and an increased number of undifferentiated gonocytes (+53%, p < 0.001) compared to chicks obtained from native semen (control group, n = 20). A decrease in the expression of DMRT1 and TGFB2 in the gonads of embryos (-48% and -29%, respectively, p < 0.05) and day-old chicks (-12% and -43%, p < 0.001 for TGFB2) was found, accompanied by an inversion of microRNA dynamics: miR-6701-3p was decreased and miR-301a-5p was increased. The obtained data provide important evidence of transgenerational effects in birds and contribute to the search for solutions to problems associated with maintaining sperm quality after cryopreservation, and indicate that cryopreservation does not simply reduce the level of molecular activity, but disrupts the ontogenetic regulatory program embedded in the genome.
Euthanasia in companion animal practice represents one of the most emotionally and ethically challenging decisions in veterinary medicine, requiring clinical judgment, effective communication, and sensitivity toward both animal welfare and pet owner well-being. This mixed-methods exploratory study investigated decision-making in small animal euthanasia from both pet owner and veterinarian perspectives. An online questionnaire completed by 228 pet owners from 17 countries was supplemented by two semi-structured interviews involving small animal veterinarians with different professional backgrounds. The qualitative interview component was exploratory and was used to contextualize the survey findings. Quantitative data were analyzed using descriptive and inferential statistics, while qualitative responses were examined thematically. Illness (63.3%) and age-related decline (31.6%) were the most frequently reported reasons for euthanasia. Most pet owners assessed their animal's condition through personal observation (53.5%) or veterinary advice (39.2%), whereas structured quality-of-life tools were rarely used (7.3%). Emotional attachment represented the most influential factor in decision-making (69.3%). Pet owners who explicitly reported receiving emotional support from their veterinarian experienced significantly lower emotional burden (Holm-adjusted p = 0.002) and greater satisfaction with communication (Holm-adjusted p = 0.006) than those who explicitly reported no emotional support. Interview findings emphasized medical justification, individualized communication, and ethical responsibility. These findings highlight the central role of communication, emotional support, and structured end-of-life guidance in improving companion animal euthanasia decision-making. Because the study relied on voluntary online recruitment and included a limited qualitative sample, the findings should be interpreted as exploratory.
To screen high-quality porcine-derived lactic acid bacteria for swine production, this study compared growth performance, acid production, acid and bile salt tolerance, and genome characteristics of Lactobacillus plantarum (MRS002), Lactobacillus amylovorus (MRS003), and Lactobacillus salivarius (MRS004). All experiments were performed with three biological replicates, and data were analyzed using the GraphPad Prism software 8.4.3 by one-way analysis of variance (one-way ANOVA) followed by Tukey's multiple comparison test (significance level p < 0.05). All three strains showed typical anaerobic growth. L. amylovorus MRS003 had a longer growth cycle and higher biomass, while L. plantarum MRS002 and L. salivarius MRS004 grew faster and produced more acid, with pH values reaching 4.2 and 4.3 at 24 h, respectively. L. plantarum MRS002 and L. salivarius MRS004 also exhibited higher survival rates under 0.3% bile salt and pH 2.0 stress. Genome annotation revealed that more than 50% of genes were related to metabolism in all strains. L. plantarum MRS002 possessed the most comprehensive metabolic and stress-resistance gene networks; L. amylovorus MRS003 shows genomic enrichment in starch-degradation pathways and appears promising for high-starch feed fermentation; and L. salivarius MRS004 showed unique advantages in aromatic amino acid metabolism (e.g., phenylalanine, tyrosine, and tryptophan biosynthesis). In summary, based on the evaluated in vitro indicators, L. salivarius MRS004 has more favorable phenotypic characteristics, L. plantarum MRS002 has broad adaptability, and L. amylovorus MRS003 is suitable for high-starch feed fermentation. This study provides a theoretical basis for the research and development of probiotic products.
Silicon (Si) is an essential trace element involved in multiple physiological processes of animals. This study aimed to investigate the dose-dependent effects of dietary silica (SiO2) supplementation on production performance and key blood parameters in laying hens. A total of 360 hens were randomly assigned to five groups (6 replicates/group, 12 hens/replicate) and fed basal diets supplemented with 0% (control), 0.1%, 0.2%, 0.4%, or 0.8% SiO2 for 8 weeks. Laying performance, egg quality, serum immune indices, reproductive hormone levels, antioxidant status, and serum trace element concentrations were determined. The results showed that dietary SiO2 supplementation significantly affected egg production rate (p < 0.05), with the 0.2% group achieving the highest rate compared to the control. For egg quality, yolk weight and yolk thickness were significantly reduced only in the 0.8% group (p < 0.05), while other parameters were unaffected (p > 0.05). Dietary supplementation with 0.2%, 0.4%, and 0.8% silica significantly increased serum levels of IL-2 and IL-4 (p < 0.05), whereas the 0.8% supplementation decreased IL-1 levels (p < 0.05). Compared with the control group, serum IgA and IgG levels were elevated in the 0.2%, 0.4%, 0.8% silica-supplemented groups (p < 0.05), and serum IgM levels were higher in the 0.4% and 0.8% groups (p < 0.05). Regarding reproductive hormones, dietary SiO2 significantly increased serum concentrations of β-endorphin, estradiol, growth hormone, luteinizing hormone, and progesterone (p < 0.05), with follicle-stimulating hormone elevated in the 0.4% and 0.8% groups (p < 0.05). Dietary silica supplementation did not affect serum activities of SOD, GSH-Px, CAT, or T-AOC. Serum POD activity decreased gradually and was significantly lower in the 0.2%, 0.4%, and 0.8% groups than in the control group (p < 0.05). Furthermore, SiO2 supplementation significantly altered serum Cu and Zn levels (p < 0.05), with the 0.8% group having the highest Ca concentration and the 0.1-0.8% groups showing increased Zn levels compared to the control; no effects on Fe and Mn were observed (p > 0.05). In conclusion, dietary supplementation with 0.2-0.4% SiO2 effectively improves egg production rate, along with enhancing immune function, modulating reproductive hormone secretion, and regulating serum Cu/Zn homeostasis in late-phase laying hens.
Despite the widespread use of phytase in swine diets, there is a need to update the current evidence on its effects on performance, carcass traits, and economic outcomes. This meta-analysis evaluated the impact of phytase supplementation on growth performance, carcass traits, and economic returns in growing-finishing pigs, combining meta-analysis with economic modeling. A systematic review (Web of Science, ScienceDirect, PubMed, and Scopus) revealed 1049 studies, 17 of which met the inclusion criteria (PRISMA). Three treatments were considered: a basal diet (BD), a reduced-P diet without phytase (DRP), and a reduced-P diet with phytase (DRP + P), with effect sizes expressed as weighted mean differences (WMDs). The meta-analysis included performance traits (daily feed intake, DFI; daily weight gain, DWG; feed conversion, FC; and final weight, FW) and carcass traits (carcass weight, CW; carcass yield, CY; backfat thickness, BT; loin eye area, LEA; lean meat percentage, LMP; and lean meat yield, LMY). With respect to BD vs. DRP, P reduction impaired performance and decreased ADG, DFI, and final weight (p < 0.001), especially at reductions ≥0.12%. In DRP + P vs. BD, phytase had no significant effects on performance (WMD = 0.008 kg/day) or carcass traits. In DRP + P vs. DRP, phytase improved performance (ADG: +0.068 kg/day; DFI: +0.106 kg/day; FW: +4.630 kg; FC: -0.115; p < 0.001), with stronger effects in males and at greater P reductions. In DRP + P vs. DB, carcass traits were not significantly affected, whereas DB vs. DRP reduced LEA (WMD = -1.820, p < 0.00), and DRP + P vs. DRP produced subgroup-dependent changes in CW, LEA, and LMY according to sex, phytase source, and diet composition. Economically, phytase increased profit by up to US$ 2.66 (vs. BD) and US$ 5.14 (vs. DRP), remaining advantageous even with enzyme price increases of up to 4×. Overall, phytase supplementation improved performance and economic returns, representing an effective strategy for the development of swine production systems.
Shearing is a stressful procedure for sheep, combining isolation, restraint, and the mechanical action of shearing, which activates the hypothalamic-pituitary-adrenal axis and induces oxidative stress. This study investigated whether melatonin-a pleiotropic hormone with well-documented antioxidant properties-administration could modulate the stress response and oxidative stress in Sarda sheep during shearing. Forty lactating ewes (aged 3-5 years, mean body weight 41 ± 1.1 kg) were randomly assigned to four groups (n = 10 each): two groups received a subcutaneous melatonin implant (18 mg); two remained untreated as controls. Within each category, one group was shorn and the other subjected only to restraint. Blood samples were collected before, during, and after shearing to measure cortisol, glucose, reactive oxygen metabolites (ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSI). Procedures elevated cortisol, glucose, ROMs, and OSI in all groups, but melatonin treatment significantly reduced these parameters and increased BAP relative to untreated animals at all sampling points (p < 0.05). No significant differences were observed between shorn and unshorn animals within the same treatment, suggesting that the handling, restraint, and isolation associated with the shearing procedure represent the major sources of stress, rather than the mechanical act of shearing itself. In conclusion, melatonin administration blunts the stress response and reduces oxidative stress in sheep during routine shearing-related handling procedures, suggesting its potential as a practical tool to improve animal welfare during routine management practices.
Seasonal human influenza A viruses (H1N1 and H3N2) are frequently transmitted to swine populations. Continued transmission of these viruses among swine results in genetic and phenotypic changes, creating new lineages of swine influenza viruses that differ from their parent strains. This contributes to increased viral genetic diversity and the emergence of zoonotic risks. The aim of this study was to assess the prevalence of influenza viruses, including presumably human-origin A(H3N2) strains, on swine farms in Kazakhstan from 2022 to 2025. Real-time RT-PCR was used to test nasal swab antigens, and hemagglutinin inhibition test, and ELISA were used to determine serum antibody levels. Influenza A/H1N1 and A/H3N2 viruses, as well as the A(H7) hemagglutinin genes, were detected in swine. Serological analysis confirmed the presence of antibodies to the A/H1N1pdm09 and A/H3N2 viruses. The influenza A(H3N2) isolates were found to be genetically similar to human viruses of the J.2 or 3C.2a1b.2a.2a.3a.1 clade, possibly indicating transmission from humans to animals. The ongoing exchange of influenza viruses between humans and swine poses a threat of future pandemics. These studies highlight the need for continuous monitoring of swine influenza in Kazakhstan to understand virus evolution and protect public health.
The range of food resources consumed by opportunistic carnivores is shaped by habitat-specific availability and productivity. However, baseline data on the diet of the Japanese weasel (Mustela itatsi) in managed wetland habitats remain scarce. Firstly, we conducted a diet study examining Japanese weasel scats from the Watarase-yusuichi wetland (WYW) (September 2024-August 2025). Based on 103 fecal samples, we calculated seasonal frequency of occurrence (FO) for 16 food categories and estimated their relative volume (RV) using the point-frame method. Surprisingly, plant material and fruit seeds were eaten frequently across seasons, and fruit seeds constituted a major component of scat volume, particularly in summer. Secondly, we compared baseline WYW data with published datasets from two contrasting aquatic habitats (a suburban river area and a rice paddy landscape) using harmonized categories and multivariate analyses. Diet composition differed markedly among study sites, and the PERMANOVA analysis indicated a significant effect of sampling site (R2 = 0.643, p = 0.001) with a weaker seasonal effect (R2 = 0.208, p = 0.038), with no significant pairwise differences between seasons (all p ≥ 0.300). Although cross-site comparisons should be interpreted cautiously because datasets were collected in different decades (1998, 2018, and 2024-2025), these findings support habitat-associated trophic plasticity in the Japanese weasel, with dietary shifts across habitats and seasons apparently reflecting variation in available food resources. In managed floodplain wetlands such as WYW, maintaining a mosaic of reed beds, ponds, and channels may help sustain dietary flexibility and population persistence in this species.
Cetacean mortality serves as a critical indicator of marine ecosystem health, reflecting the cumulative impacts of climate-driven environmental shifts and anthropogenic pressures. However, long-term national-scale assessments remain limited in Thailand. This study aimed to assess population status, analyse spatiotemporal mortality patterns, and evaluate the influence of environmental drivers and anthropogenic stressors in Thai waters over the past two decades. Secondary data from multiple sources were analysed using generalised linear models with a negative binomial distribution. A total of 29 cetacean species were recorded, with an estimated population of approximately 3000 individuals. Mortality was documented in 24 species and showed an increasing trend over time. Coastal species, particularly Irrawaddy dolphins and finless porpoises, accounted for the majority of deaths (56%). Mortality patterns varied significantly by region (p < 0.05) but not by season, with the highest levels observed in the Upper Gulf of Thailand. Environmental factors were significantly associated (p < 0.05) with mortality, including wind speed in the Andaman Sea and extreme conditions (drought and heavy rainfall) in the Upper Gulf. In the Lower Gulf of Thailand, mortality was significantly associated with a combination of environmental (sea surface temperature and wind speed) and anthropogenic factors (fishery production). Overall, environmental variability appeared to exert a stronger influence than anthropogenic stressors. These findings highlight the requirement for targeted monitoring in high-risk regions and periods, alongside improved investigation of mortality causes to support effective conservation strategies.