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This study used a between-subjects experimental design to examine how five spoiler conditions, including a complete scene without spoiler, a chaotic scene without spoiler, a video spoiler, a verbal spoiler, and a written spoiler, influenced viewers' narrative engagement, heart rate synchrony measured by inter-subject correlation, and task performance while watching suspenseful film clips. Seventy-six university students were randomly assigned to one of the spoiler groups and viewed clips from the film Revenge. Narrative engagement was assessed using the Narrative Engagement Scale (NES), and ECG signals were recorded to derive heart rate time series for ISC-HR analyses. Key cue search task was indexed by a sticker-counting task during viewing. Results indicated that the video spoiler group showed the most consistent advantages, including higher narrative engagement, more frequent key cue search task, and the highest ISC-HR during the spoiler exposure phase. In contrast, the chaotic scene without spoiler group showed the weakest subjective engagement profile, with differences most evident in comprehension-related engagement and partially attentional focus. ISC-HR varied by spoiler modality primarily during the spoiler exposure phase, with the video spoiler group eliciting the highest synchrony, whereas ISC-HR during movie watching did not show clear group differences. Overall, these findings suggest that spoiler modality and narrative coherence shape viewers' cognitive processing and cue monitoring during film viewing, and that ISC-HR can be used as a complementary physiological indicator to support the interpretation of immersion-related self-reports by indexing shared response dynamics among viewers.

Understanding how we perceive and interpret the movements of other living beings is fundamental to social interaction, survival, and navigating our dynamic environment. Traditional studies of biological motion perception have predominantly employed simplified stimuli, such as point-light displays, which sacrifice ecological validity for experimental control. To overcome this limitation, we created a novel stimulus set featuring naturalistic videos of isolated hand and leg movements. These stimuli incorporated variations in viewing perspective (first-person vs. third-person) and the presence or absence of object interactions, thereby increasing their real-world relevance. We recorded electroencephalography (EEG) from 28 adult participants as they viewed these goal-directed movements alongside nature scenes that served as control stimuli. Neural engagement was assessed through two complementary measures: inter-subject correlation (ISC), which captures shared neural responses across viewers, and intra-subject correlation (IaSC), which reflects temporal consistency of responses within individual viewers. We hypothesized that (1) biological limb movements would elicit stronger neural synchrony than non-biological motion, (2) movement type (arm vs. leg), object interaction, and visual perspective would differentially modulate ISC based on distinct affordance-related processing systems, and (3) these effects would replicate upon second exposure. Our findings revealed that lower-limb movements elicited significantly greater ISC compared to both upper-limb movements and nature scenes. This effect proved robust, replicating consistently when participants viewed the same experimental conditions a second time. Furthermore, specific stimulus features modulated neural synchronization in domain-specific ways: first-person perspective enhanced ISC specifically for leg videos, whereas object interaction increased ISC specifically for arm videos. Notably, IaSC showed no differences across conditions, revealing a dissociation between cross-individual neural alignment and within-individual temporal consistency. These findings provide new insights into how particular features of naturalistic movement systematically modulate shared neural engagement, advancing our understanding of biological motion perception in ecologically valid contexts. The dissociation between ISC and IaSC suggests that cross-individual neural synchronization and within-individual temporal consistency reflect distinct neural processes.

With the rise of highly visual social platforms such as Xiaohongshu, women are increasingly susceptible to the negative influences of idealized appearance images, and focusing on the underlying protective mechanism is of great significance. The interactive nature of such platforms may also play a protective role: content posters can interact with others through self-disclaimers, while viewers can also respond by comments, whose effects on women's body dissatisfaction need further examination. Therefore, this research conducted two experimental studies (Study 1: N = 106; Study 2: N = 92; aged 17-25 years) using 3 × 2 mixed designs to examine the roles of different types of self-disclaimers (study 1) and comments (study 2), respectively. The findings indicated that elaborated self-disclaimers or critical realism comments could play a protective role, buffering the negative influences of idealized appearance images; while brief self-disclaimers and positive appearance comments have no protective effect, which lead to the same degree of increased body dissatisfaction as viewing pure idealized images. These findings could deepen our understanding of the mechanisms underlying the effects of idealized appearance images and body dissatisfaction, providing guidance for body image interventions and media literacy education.

School bullying is a pervasive global concern that profoundly affects teenagers' development and mental health. This study examines the influence of physical exercise on bystander intervention in bullying, analyzing the psychological mechanisms that underpin this relationship through the frameworks of embodied cognition and mind-body interaction theories. The study aims to determine if physical exercise facilitates bystander intervention in school bullying by improving physical self-efficacy and a sense of justice. In 2024, a cross-sectional survey was administered with 954 middle school students (566 males, 388 females; mean age 13.85&#x202f;years) in Jiangxi, China. Participants completed validated instruments measuring physical exercise, self-efficacy, sense of justice, and bystander intervention behavior. Data were analyzed utilizing SPSS and AMOS structural equation modeling (SEM) to evaluate the correlation between physical exercise and bystander conduct, along with the mediating effects of physical self-efficacy and sense of justice. A duration of 8&#x202f;weeks involving martial arts with 51 male pupils was employed to investigate potential causal links. A Repeated-Measures ANOVA was performed to assess the impact of the intervention. Structural equation modeling indicated that physical exercise strongly predicts bystander intervention behavior (&#x3b2;&#x202f;=&#x202f;0.124, p&#x202f;<&#x202f;0.01), with physical self-efficacy (&#x3b2;&#x202f;=&#x202f;0. 118, p&#x202f;<&#x202f;0.01) and sense of justice (&#x3b2;&#x202f;=&#x202f;0.095, p&#x202f;<&#x202f;0.01) acting as mediators. The intervention outcomes indicated enhancements in physical self-efficacy and sense of justice, resulting in a rise in bystander intervention. These findings indicate that physical exercise can significantly facilitate the transition of bystanders from passive viewers to proactive helpers, providing essential insights for the mitigation and prevention of school bullying.

Adapting musical theater into film involves the mediation of performance language from stage to screen. While stage language depends on live singing, spatial ensemble, and performer-audience co-presence, screen language relies on framing, editing, and audiovisual control. Although musical theater film adaptation has attracted increasing scholarly attention, audience-based research on how this transformation is perceived remains limited, especially in Chinese and Anglo-American contexts. This study examines how stage language is transformed into screen language through four stage-to-screen cases: the musical theater Les Mis&#xe9;rables, first staged in 1980, and its 2012 film adaptation; the musical theater Dear Evan Hansen, first staged in 2017, and its 2021 film adaptation; the original Chinese musical theater Jinsha, first staged in 2005, and its 2023 film adaptation; and the original Chinese musical theater The Long Night, first performed in 2021, and its 2022 screen version. Guided by the Cultural Adaptability Symbolic Stratification (CASS) model, the study adopts a mixed-methods approach combining case analysis, content analysis, questionnaires, and focus group discussions with 27 participants. The findings identify three pathways of transformation: Cinematic Translation, Direct Transplantation, and Fragmented Reconfiguration. Among them, Cinematic Translation generated the strongest audience engagement, especially in emotional immersion and narrative clarity. Direct Transplantation preserved visible stage conventions but often weakened narrative and emotional coherence. Fragmented Reconfiguration produced more variable responses, depending on viewers' interpretive expectations and familiarity with non-linear form. Some age-related tendencies also appeared within this sample, but these should be treated cautiously given the limited number of middle-aged and older participants. Overall, the study shows that successful adaptation depends less on preserving theatrical form than on cinematically reorganizing performance language. It also refines the application of the CASS model to performance analysis and offers practical insight into the adaptation of Chinese original musical theater works into musical theater film.

Lumbar spinal stenosis (LSS) is a degenerative disorder in which narrowing of the spinal canal compresses neural elements, causing pain, numbness, and limited mobility. With the rapid growth of Chinese short video platforms (TikTok, Bilibili, Xiaohongshu, Kwai, and WeChat), the public increasingly relies on short videos for LSS-related health information. However, the quality of such content has not been systematically evaluated. This cross-sectional content analysis searched each of five platforms using "lumbar spinal stenosis," screened the top 100 results, and included 412 videos after applying predefined criteria. Basic characteristics and engagement metrics were extracted. Analyses were stratified by platform, uploader type, and video category. Video quality and reliability were assessed using the Global Quality Score (GQS), modified DISCERN (mDISCERN), and JAMA benchmark criteria. Spearman correlation analysis examined associations between video characteristics and quality scores. High-quality content was concentrated on Bilibili and WeChat, particularly scientific explanations and professional course videos uploaded by healthcare professionals, whereas Kwai showed consistently low GQS scores across uploader types and categories. Video duration was moderately and positively correlated with GQS (r&#x2009;=&#x2009;0.423), mDISCERN (r&#x2009;=&#x2009;0.340), and JAMA scores (r&#x2009;=&#x2009;0.357; all p&#x2009;<&#x2009;0.001). Follower count and most engagement metrics (likes, saves, shares) showed only weak correlations with quality. Overall, the quality and reliability of LSS-related short videos on major Chinese platforms are suboptimal, with marked inter-platform variation. Content from non-professional uploaders and personal experience-focused videos tended to be of lower quality. Healthcare professionals and medical institutions should actively disseminate evidence-based LSS information via short video platforms, and viewers should preferentially seek credible, verifiable sources.

Visual attention in interpretive settings is shaped not only by image saliency but also by the context viewers receive before viewing. However, how different context formats relate to attention allocation in curated visual scenes remains under-specified, and findings are often sensitive to the evaluation metric used. We conducted an exploratory free-viewing eye-tracking study in which participants viewed keyframes from museum-oriented scenes after brief pre-viewing context presented in different formats. The dataset comprises gaze recordings from 10 participants across 30 images. Attention patterns were evaluated by comparing observed gaze distributions with a saliency-model baseline and summarizing correspondence through a multi-metric profile. The results indicate systematic block-level differences in gaze-reference correspondence and variability, suggesting that pre-viewing context can be reflected in where attention concentrates, while different metrics highlight different aspects of deviation. These findings offer a compact, metric-aware way to report context-associated attention differences in small-sample studies and inform interpretive design decisions where textual and graphical cues are used to shape viewing.

With the increasing use of digital platforms in surgical education, YouTube has become a widely accessible resource for trainees. However, the absence of peer review raises concerns regarding the reliability and educational value of its content. This study aimed to evaluate the educational quality, reliability, and instructional value of inguinal hernia repair videos on YouTube using multiple validated scoring systems. A systematic search was conducted on YouTube, and 50 videos meeting predefined inclusion criteria were analyzed. Videos were independently assessed by two blinded reviewers using the Global Quality Scale (GQS), Journal of the American Medical Association (JAMA) criteria, Video Power Index (VPI), Laparoscopic Video Educational Guidelines and Scoring (LAP-VEGaS), DISCERN, and Health on the Net (HONcode) criteria. Videos were also categorized according to their source. Of the 50 videos, 42% were uploaded by individual users and 36% by academic or institutional sources. Most videos demonstrated laparoscopic or robotic procedures. Median scores indicated moderate educational quality (GQS 3, JAMA 3, LAP-VEGaS 11). Videos categorized as originating from academic or institutional sources tended to achieve higher scores; however, these findings should be interpreted with caution. No significant correlation was found between video popularity (VPI) and educational quality. YouTube provides a widely accessible but variable educational resource for inguinal hernia surgery, with overall moderate quality even among selected videos. Video popularity alone does not reliably indicate educational value. Instead, viewers may benefit from prioritizing videos with structured step-by-step narration, clear visualization of key anatomical landmarks, transparent source identification, and inclusion of complication management. While YouTube may support learning as a supplementary tool, it may not adequately replace structured surgical training.

The public transportation system is a high-risk medium for respiratory infectious disease (RID) transmission. Health awareness videos are integral to disseminating knowledge, elevating health-risk perceptions, and enhancing attitudes toward precautionary measures against RID, as per the Health Belief Model and Cognitive Dissonance theory. This study aimed to produce and evaluate an evidence-based health awareness video on precautionary measures against RID among public transportation drivers (PTDs) in Lebanon. This is a multistage project executed over 2 fiscal years. In stage 1, a scoping review study identifies articles on RID among PTDs. Search strategy follows a combination of concepts (influenza, COVID-19, and public transportation) using PubMed, CINAHL Complete, and other databases up to September 2025. Studies that reported the characteristics of PTDs, their knowledge, perceptions, and compliance with precautionary measures against RIDs are reviewed. In stage 2, based on the scoping review, a script for a health-awareness video is drafted. Using a classic Delphi technique, the script is revised and approved by 8 experts in public health, infection control, psychology, and filmmaking, using the Patient Education Materials Assessment Tool for audiovisuals (PEMAT-AV), and DISCERN. PEMAT-AV (13-items) systematically evaluates the understandability and actionability of educational materials. DISCERN tool (16-items) evaluates the quality of consumer health information. In stage 3, a nonblinded, randomized, 2-arm controlled trial is conducted on 387 drivers from different regions. Using a simple randomization technique, the experimental group watches the new video. The control group watches a neutral video on traffic signs. To evaluate the effectiveness of the video, participants' knowledge, risk perceptions, attitudes, and cognitive dissonance are measured using infection control guidelines, Health Belief Model tool, Vaccination Attitudes Examination scale, and dissonance thermometer. Using IBM SPSS, between-group analysis is performed with Student t test, ANOVA, and Pearson correlation, followed by forward-stepwise linear regression analyses (2-sided &#x3b1;=.025). In stage 1, 20 relevant studies were identified. Studies from Lebanon described the sociodemographics, health- and work-related characteristics, and life challenges of the target population. Other studies reported influenza or COVID-19 vaccine hesitancy, low vaccine uptake, inadequate cleaning or disinfection of vehicles, and elevated risk perceptions among computers. The prevalence and impact of RIDs and determinants of precautionary measures against RIDs were identified. In stages 2 and 3, the video will be revised by experts (projected 5 months), produced (projected 6 months), and tested (projected 2 months). The study has not been funded yet. Findings from the scoping review study inspired the writing of the script (characters and dialogue), making it evidence-based and tailored toward the target viewers. A health awareness video on precautionary measures against RIDs contributes to a safer public transportation system and higher service usage. It aligns with the global mission to control the spread of RID and to lessen the disease burden.

This study aimed to assess the quality and reliability of health information in the 100 most-viewed YouTube videos related to semaglutide for weight loss, as of December 2024. The study also explored the relationship between engagement metrics and content quality, with attention to the prevalence of misinformation. A cross-sectional evaluation was conducted in December 2024. The top 100 English-language YouTube videos retrieved using the search term "semaglutide weight loss" were analyzed. Each video was assessed using 2 validated tools: the Global Quality Score and the Modified DISCERN (quality assessment tool for consumer health information) instrument. Viewer engagement data - including likes, comments, and views - were recorded. Statistical analyses included descriptive statistics and multiple linear regression to examine relationships between engagement metrics and content quality. Videos from academic and healthcare-affiliated sources generally scored higher in quality assessments, while those produced by individual users tended to lack source citations and balanced information. Although certain engagement metrics, such as the number of likes and comments, showed modest associations with higher Global Quality Scores, view count did not consistently predict quality. A notable portion of user-generated videos lacked discussion of semaglutide's risks and contraindications. The study highlights the variability in quality among semaglutide-related videos on YouTube. Engagement does not necessarily reflect the reliability of content, underscoring the importance of guiding viewers toward credible health sources. Enhancing digital health literacy and promoting greater visibility of evidence-based content may help improve the quality of health information encountered on widely used digital platforms.

Metabolic dysfunction-associated steatotic liver disease (MASLD), causing cirrhosis and liver cancer, is prevalent worldwide. Analyses of internet searches for "fatty liver" and methods to raise MASLD awareness are needed. We retrospectively analyzed co-occurring terms with "fatty liver" and tracked search histories to identify key terms. In a comparative study, a banner advertisement about MASLD was shown to individuals searching for key terms (key term group: KTG) or regardless of the search terms (control group: CTR). The ad redirected viewers to an educational cartoon, followed by a questionnaire. The most frequent co-occurrence term was "how to treat." People searched mainly for lifestyle-related disease and diet terms. Key terms included "diet," "blood sugar level," "cholesterol," "triglyceride," "visceral fat," and "antihypertensive drugs." Banner views were 5&#x2009;864&#x2009;184 for KTG and 49&#x2009;388&#x2009;176 for CTR. KTG had a higher click-through rate than CTR (0.097% vs. 0.063%; p&#x2009;<&#x2009;0.001) and more survey responses (3.22% vs. 0.84%; p&#x2009;<&#x2009;0.001). KTG participants also had a greater intention to seek medical care early (60.2% vs. 42.4%; p&#x2009;=&#x2009;0.003). Search term-based advertising may help increase public awareness of MASLD and may encourage earlier intention to seek medical consultation.

To demonstrate retroperitoneal sentinel lymph node dissection for early-stage cervical cancer using vaginal natural orifice transluminal endoscopic surgery (vNOTES). Lymph node status plays a central role in staging and treatment planning in cervical cancer [1]. In early-stage disease, confirmation of nodal involvement may alter management from primary surgery to chemoradiotherapy [2]. Sentinel lymph node biopsy has emerged as a minimally invasive and reliable method for nodal assessment [3]. This video article demonstrates retroperitoneal sentinel lymph node dissection via vNOTES in 7 surgical steps. About 56-year-old woman with cervical adenocarcinoma INTERVENTIONS: We present the case of a 56-year-old woman with cervical adenocarcinoma and a radiologically suspicious left pelvic lymph node measuring approximately 1 cm on preoperative magnetic resonance imaging. Because suspected nodal involvement would directly influence treatment planning, sentinel lymph node sampling was performed before definitive surgery. To improve orientation for viewers, the text includes a literal drawing of the pelvic retroperitoneal pathway and the expected location of the sentinel lymph node, together with expanded narration emphasizing key anatomic landmarks and dissection steps (Fig. 1). Although indocyanine green may provide higher detection rates, isosulfan blue was used because indocyanine green was not available at our institution. A total of 2 mL of undiluted isosulfan blue was injected into the cervix at the 3 and 9 o'clock positions, with 0.5 mL injected superficially and 0.5 mL deeply at each site. The procedure consisted of: (1) cervical blue dye injection; (2) entry into the retroperitoneal space through the lateral vaginal fornix; (3) retroperitoneal dissection; (4) identification of anatomic landmarks; (5) detection of the sentinel lymph node; (6) obturator fossa dissection; and (7) sentinel lymph node excision. Retroperitoneal sentinel lymph node dissection using vNOTES appears feasible as a minimally invasive approach for nodal assessment in selected patients with early-stage cervical cancer and may support individualized treatment planning while avoiding abdominal access. VIDEO ABSTRACT.

This study investigates how chromatic manipulation of cinematic content modulates emotional engagement, with specific attention to sex-differentiated responses. We used a mixed factorial design with chromatic condition as a within-subject factor and biological sex as a between-subject factor, counterbalanced across scenes through a 3 &#xd7; 3 Latin square that renders scene identity orthogonal to chromatic condition by construction. Thirty adult viewers were recorded with synchronised facial-expression analysis (AFFDEX 5.1), blink detection, and galvanic skin response (Shimmer GSR). The primary inferential target was the Condition &#xd7; Sex interaction on automated positive facial valence. This interaction was statistically reliable under three converging tests: a mixed-effects model (&#x3b2;Mod&#xd7;F=-4.48, SE=2.16, 95% CI [-8.81,-0.14], p=0.043), a participant-level cluster bootstrap (2000 resamples; 95% percentile CI [-9.78,-0.63]; pboot=0.011), and a label-permutation test. The effect was stable under leave-one-subject-out resampling (100% sign-stability) and persisted after introducing scene as a fixed factor. Blink rate and electrodermal activation showed directionally consistent but weaker interaction patterns. A multidimensional engagement framework that separates attentional-autonomic intensity from expressive valence supports interpretation of the finding as specific to expressive affective behavior rather than to overall activation. The results provide empirical evidence that chromatic manipulation in realistic cinematic stimuli modulates expressive affective responses in a sex-dependent manner, and they establish a reproducible multimodal biometric framework for chromatic impact assessment.

Visual art, as a non-verbal medium for emotional expression and regulation, demonstrates unique therapeutic potential in mental health intervention. However, existing research lacks an integrated theoretical framework to systematically explain how the visual characteristics of artworks influence viewers' emotional responses. This study aims to construct and validate the "Theme-Color-Emotion" (TCE) theoretical framework, revealing the interactive mechanisms between thematic content, color characteristics, and emotional responses to visual artworks. Based on the WikiArt Emotions dataset (n&#x202f;=&#x202f;4,105 artworks) and the ArtEmis dataset (455&#x202f;K emotion annotations), chi-square tests, correspondence analysis, and multidimensional cross-analysis methods were employed to systematically examine the association patterns between theme types, color characteristics, and emotional responses. Artworks with natural landscape themes were significantly more associated with positive emotional responses than other theme types (67.3% vs. average 52.1%, &#x3c7; 2&#x202f;=&#x202f;234.7, p&#x202f;<&#x202f;0.001). Cool colors (blue, green) clustered with calm and contemplative emotions, while warm colors (red, orange, and yellow) clustered with pleasant and exciting emotions. The TCE framework achieved 87.6% accuracy in classifying artworks according to viewer-reported emotional valence, a 9.3 percentage point improvement over the single-color baseline model. Operationalized through a logistic regression model integrating thematic and chromatic features, the TCE framework reveals that theme and color jointly account for viewer-reported emotional valence in ways that neither dimension captures in isolation. These findings provide a systematic analytical perspective for understanding the associations between visual artwork characteristics and viewer emotional responses, offering evidence-based implications for therapeutic art design and art therapy practice.

Poster presentations play a vital role in the dissemination of scholarly work and provide key opportunities for networking and professional growth. However, suboptimal poster design often hinders this transfer of knowledge and opportunity for meaningful engagement with peers. Drawing from principles of visual design and the increasing prominence of the "Better Poster" format, this Perspectives article presents practical suggestions for designing posters that hone messaging and promote interaction with viewers. We recommend a shift away from thinking of posters as a medium to present every detail of one's scientific work and toward reimagining posters as a tool to promote scholarly dialogue.

Traumatic brain injury (TBI) remains a leading cause of neurological morbidity and mortality, characterized by complex pathophysiological cascades. Here, we investigate the role of the transcription factor EGR1 (early growth response 1) in modulating mitochondrial homeostasis via the HIF1A (hypoxia inducible factor 1, alpha subunit)-BNIP3 (BCL2/adenovirus E1B interacting protein 3) axis following TBI. Using integrated transcriptomic and epigenomic analyses, we identified EGR1 as a critical regulator of TBI pathology, with its expression acutely upregulated in neurons post-injury. Genetic ablation of Egr1 in mice significantly reduced neuronal apoptosis, preserved dendritic integrity, and ameliorated cognitive and sensorimotor deficits. Mechanistically, chromatin immunoprecipitation and luciferase assays revealed that EGR1 directly binds to the Hif1a promoter, repressing its transcription. Loss of EGR1 enhanced HIF1A-BNIP3-mediated mitophagy, reducing mitochondrial dysfunction and oxidative stress both in vitro and in vivo. Conversely, silencing HIF1A or BNIP3 abrogated the neuroprotective effects of EGR1 deficiency. These findings establish a novel EGR1-HIF1A-mitophagy signaling axis as a key determinant of TBI outcomes, highlighting EGR1 as a potential therapeutic target. Abbreviations: AAV: adeno-associated virus; ACTB/&#x3b2;-actin: actin, beta; AIF1/IBA1: allograft inflammatory factor 1; BAF: bafilomycin A1; BNIP3: BCL2/adenovirus E1B interacting protein 3; CCI: controlled cortical impact; COX8: cytochrome c oxidase subunit 8; CUT&Tag: cleavage under targets and tagmentation; DAPI: 4,'6-diamidino-2-phenylindole; DEGs: differentially expressed genes; eGFP: enhanced green fluorescent protein; EGR1: early growth response 1; GFAP: glial fibrillary acidic protein; GO: gene ontology; GSEA: gene set enrichment analysis; HCQ: hydroxychloroquine; HIF1A/HIF-1&#x3b1;: hypoxia inducible factor 1, alpha subunit; IGV: integrative genomics viewer; KEGG: Kyoto encyclopedia of genes and genomes; KO: knockout; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; Lv: lentivirus; MAP2: microtubule-associated protein 2; mCherry: monomeric cherry fluorescent protein; mRFP: monomeric red fluorescent protein; MTOR: mechanistic target of rapamycin kinase; MUT: mutant; MWM: Morris water maze; NAB1: Ngfi-A binding protein 1; NAB2: Ngfi-A binding protein 2; RBFOX3/NeuN: RNA binding protein, fox-1 homolog (C. elegans) 3; OGD: oxygen-glucose deprivation; OLIG2: oligodendrocyte transcription factor 2; PBS: phosphate-buffered saline; PECAM1/CD31: platelet/endothelial cell adhesion molecule 1; PFA: paraformaldehyde; PPI: protein-protein interaction; Puro: puromycin; ROI: region of interest; ROS: reactive oxygen species; SEM: standard error of the mean; SQSTM1/p62: sequestosome 1; TBI: traumatic brain injury; TOMM20: translocase of outer mitochondrial membrane 20; TSA: tyramide signal amplification; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; VDAC1: voltage-dependent anion channel 1; WT: wild-type.

This study explores how a shared-viewer data platform, SAMBLIK-diabetes, supports clinical supervision and cross-sector collaboration between general practitioners (GPs) and hospital specialists in the management of complex type 2 diabetes cases. A qualitative process evaluation was undertaken within a multi-regional roll-out of SAMBLIK-diabetes in Denmark. Semi-structured interviews and observations explored how using the platform's shared-view function supported telephone conferences between GPs, practice nurses, and endocrinologists. Data were analysed and categorized inductively. Out of 21 participating clinicians, 16 reported that the shared-view platform enhanced clarity, efficiency, and equity in communication. The ability to see identical patient data-such as HbA1c trajectories, medication history and renal function-fostered shared situational awareness and mutual confidence. Barriers to use included inconsistent platform use, difficulty isolating the unique contribution of the platform to care coordination, and an unresolved gap in enabling nurses to initiate hospital specialist contact. SAMBLIK-diabetes demonstrates how a shared-view platform visualizing patient data can strengthen cross-sectoral dialogue and clinical reasoning in chronic disease management. To ensure sustainability, governance clarity, seamless workflow integration, and professional training are essential.

Conventional holographic three-dimensional (3D) displays have a limited ability to enlarge their field of view (FOV) and cannot reconstruct panoramic 3D scenes. This paper proposes a method to overcome this limitation by using a concave ellipsoidal mirror. The concave ellipsoidal mirror has two focal points and can converge wavefronts from its entire circumference to one focal point. Thus, a viewer located at the focal point can capture wavefronts in all directions, thereby achieving an omnidirectional FOV. To validate the proposed concept, we developed a prototype time-division system and demonstrated panoramic 3D scene generation with an FOV exceeding 270&#x2218;, far beyond the limited FOV of conventional holographic 3D displays.

Artificial intelligence (AI) tools are increasingly used for mental health support, yet little is known about how they are understood outside clinical trials and survey-based research. This study examined public perceptions of AI mental health support through a convergent mixed-methods analysis of 7,949 YouTube comments posted across ten videos discussing AI and mental health. Quantitative analyses included VADER sentiment analysis, NRC emotion profiling, latent Dirichlet allocation topic modelling, and keyword co-occurrence network analysis. Qualitative analysis used Braun and Clarke's reflexive thematic analysis to identify patterns of shared meaning within a purposive sample of high-engagement comments. Overall sentiment was moderately positive (57.93%), but this positivity was qualified by substantial negative sentiment (24.58%) and recurring emotional signals of both trust (14.98%) and fear (7.92%). Topic modelling showed that the dominant lines of discussion centred on unmet support needs and the question of whether AI should replace human therapists. The thematic analysis generated five themes: AI as a bridge where human care is absent, AI as comforting but overly validating, AI as unable to reproduce authentic human therapeutic encounter, AI as a possible driver of social disconnection, and AI as a site of privacy and commercial concern. Taken together, the findings suggest that public responses to AI mental health tools are best understood as conditional rather than absolute. Commenters valued AI when it provided immediacy, low-cost access, and a low-risk space for disclosure, but resisted it where it appeared to threaten empathy, relational depth, or privacy. The study contributes a large-scale, naturalistic account of public reasoning about AI mental health support and highlights implications for digital mental health design, governance, and human-in-the-loop care.