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The article "Impact of Renal Artery Stenosis on Renovascular Hypertension and the Therapeutic Role of Renal Artery Stenting: A Comprehensive Review" [Kidney Blood Press Res. 2025; https://doi.org/10.1159/000545135] by Changgang Shao, Guoqing Chi, Fang Li, Hongcheng Ren, Liyan Zhang, Jinming Yang, Bin Wang and Mingchao Ding has been retracted by the Publisher and the Editor.After peer review, the accepted, unedited manuscript was published online as Early View. The authors did not respond to our requests and communication regarding the production process of their article despite extensive attempts at contact. As the article has not been approved by the authors for publication, we cannot publish the final version. To avoid confusion for readers we are retracting the Early View accepted, unedited manuscript.The authors did not respond to correspondence about the retraction.
This mixed-methods study evaluated the effectiveness of a patient-as-teacher learning model in undergraduate mental health nursing using a pre-/post-test design. Pre- to post-test stigma decreased in the intervention group (n = 42), notably in disclosure and help-seeking; there was no change in the control group (n = 41). Empathy increased substantially in both groups, with the largest gains in empathic concern; a marginally larger within-group effect was seen in the intervention group. Qualitative analysis identified 3 themes: deconstructing stigma and reshaping perspectives, integrating knowledge and practice, and cultivating self-reflection and caring attitudes. The patient-as-teacher model appears to support stigma reduction and empathy development.
Cancer is a globally devastating disease that severely threatens human health and impedes social development. Camptothecin and its derivatives serve as crucial chemotherapeutic agents in clinical cancer treatment, owing to their unique inhibitory activity against DNA topoisomerase I (TOP1). Although the post-modifications of camptothecin (e.g., hydroxylation and subsequent methoxylation) have been well elucidated, the core biosynthetic pathway of camptothecin remains largely unclear. In the present study, we report an unexpected and significant finding: strictosamide can be directly converted to pumiloside both in several plant species (Nicotiana benthamiana, Salvia miltiorrhiza, and Atractylodes macrocephala) and in vitro. We term this process the "Direct Express Train" of the camptothecin biosynthetic pathway. We further demonstrate that this direct conversion proceeds more efficiently under alkaline conditions in vitro. Intriguingly, light was found to effectively facilitate this conversion. Under light exposure, exogenous flavin adenine dinucleotide (FAD) supplementation markedly promoted the reaction, with the final conversion rate reaching 38.8%. These findings not only deepen our understanding of the camptothecin biosynthetic pathway but also provide a novel strategy for the efficient biosynthesis of pumiloside (7) using plant chassis such as N. benthamiana.
Hypertensive disorders of pregnancy (HDPs) are leading causes of maternal and fetal morbidity; yet uptake of home blood pressure monitoring (HBPM) for perinatal detection and management of hypertension remains limited by workflow and integration barriers. We designed and evaluated a clinician-facing HBPM report that integrates blood pressure trends, medication adherence, and pregnancy symptoms to support clinical adoption. Between March 2023 and October 2024, we designed and refined HBPM report prototypes through a mixed method study that included interviews with 16 obstetric providers, using iterative input from a multidisciplinary Clinician Advisory Group. Data on preferences for report features, usability, and workflow integration drawn from provider interviews were compiled and synthesized via convergent quantitative and qualitative methods. Sixteen providers (37.5% nurses, 25% residents, 25% attendings, 12.5% APPs) were interviewed. Providers identified the blood pressure graph, abnormal-value highlights, medication adherence tracker, and symptom log as the most useful features (n = 15-16, ≥90%), while the prenatal vitamin tracker was least useful (n = 9, 56%). Most providers preferred to review the report themselves (n = 11, 73%) and to receive it at a frequency based on clinical need (n = 12, 80%). While most clinicians were willing to integrate the report into their practice, they raised concerns about workflow burden, triage of abnormal readings, and EMR integration as barriers to successful integration. Providers found most elements of the HBPM tool useful for HDP management, but identified workflow and implementation challenges as key barriers, highlighting the need for strategies to support clinical integration.
Surgical decision-making for intraductal breast lesions has traditionally relied on physician expertise and imaging characteristics, lacking a comprehensive evaluation framework that integrates oncological efficacy, perioperative safety, and patient subjective experience. This study aimed to systematically synthesise the latest evidence and incorporate patient-reported outcomes (PROs) to construct a three-dimensional evaluation model, thereby providing an evidence base for the individualised selection between vacuum-assisted excision (VAE) and open surgery. A systematic comparison and descriptive analysis were performed across three dimensions: oncological efficacy, perioperative safety, and PROs. A total of 26 studies were included. Meta-analysis revealed no significant difference in residual lesion rates between the VAE and open surgery groups [risk ratios (RR) =1.02; 95% confidence interval (CI): 0.29-3.57; P=0.97]. Notably, the VAE group demonstrated a 59% significant reduction in recurrence rates (RR =0.43; 95% CI: 0.21-0.86; P=0.02). Due to the lack of direct comparative data, malignancy detection rates were presented descriptively without statistical pooling, and no inferences regarding diagnostic superiority were drawn. Regarding perioperative safety, VAE was associated with a significantly shorter operative time [mean difference (MD) =-14.38 min; 95% CI: -17.09 to -11.67; P<0.01] and reduced intraoperative blood loss (MD =-9.10 mL; 95% CI: -11.29 to -6.92; P<0.01). Furthermore, VAE significantly lowered the risks of skin ecchymosis (RR =0.43; 95% CI: 0.20-0.95; P=0.04), wound infection (RR =0.31; 95% CI: 0.14-0.69; P=0.004), and breast deformity (RR =0.19; 95% CI: 0.05-0.74; P=0.02). For PROs, patients in the VAE group reported significantly higher cosmetic satisfaction (RR =1.34; 95% CI: 1.15-1.56; P<0.001) and lower postoperative pain scores (MD =-1.61; 95% CI: -2.68 to -0.54; P=0.003). VAE offers clear benefits such as being minimally invasive, supporting postoperative recovery, and improving cosmetic satisfaction, making it a preferred method for radiologically localised lesions. However, current evidence is insufficient to confirm VAE as a definitive treatment for malignant or high-risk intraductal conditions. Open surgery remains essential to ensure oncological safety through thorough margin assessment and accurate detection of malignancy; the potential clinical consequences of missing malignancy greatly outweigh minor cosmetic outcomes. Future treatment options should be guided by personalised decision-making that considers lesion characteristics and patient preferences.
The template-based pelvic lymph node dissection (PLND) technique is an essential part of the radical nephroureterectomy (RNU) technical system. Recently, the lateral decubitus-positioned total retroperitoneal laparoscopic radical nephroureterectomy (LTRL-RNU) has been introduced. Here, we present a lateral decubitus-positioned laparoscopic PLND technique for this novel technical system. From July 2022 to September 2024, 11 patients with high-risk distal upper tract urothelial cancer (UTUC) underwent PLND during LTRL-RNU. All the surgical procedures were performed under a caudal view by the same surgeon. The surgery needed 0-1 additional trocars. The separation was practiced along the psoas muscle on the dorsal side of the external iliac artery and external iliac vein until the obturator nerve was exposed. Then, the obturator nerve, the common iliac artery, the external iliac artery and the external iliac vein were skeletonized, and the lymphatic adipose tissue was dissected. The basic characteristics and surgical outcomes were retrospectively analyzed. The mean operation time for RNU was 142.55±40.94 min, and the mean time for PLND was 31.18±8.62 min. Three to five trocars were used for the whole procedure, and 0.40±0.50 additional trocars were used for PLND. The only complication noted was one case of mild anemia (Clavien-Dindo grade I) without transfusion. One patient had severe scoliosis, two had external iliac artery tortuosity, one had common iliac artery tortuosity, and one patient experienced inferior vena cava tumor thrombus (Mayo Clinic Grading System grade I). The success rate of the surgery was 100%, and the pathological positive rate of PLND was 9.09%. PLND under LTRL-RNU is feasible, and its inclusion may allow lateral decubitus-positioned retroperitoneal laparoscopy to become a standard approach for treating distal UTUC.
Acute symptomatic seizures as the main manifestation of contrast-induced encephalopathy are relatively uncommon. A 50-year-old woman was admitted to our hospital with an unruptured right middle cerebral artery bifurcation aneurysm and underwent elective endovascular stent-assisted coiling. The interventional procedure was uneventful; however, she developed generalized tonic-clonic seizures consistent with status epilepticus on the first postoperative day. A computed tomography scan obtained on postoperative day 1 revealed mild edema in the right cerebral hemisphere, suggestive of contrast-induced encephalopathy. Following prompt antiepileptic therapy and supportive care, the patient achieved complete neurological recovery without residual deficits. By reporting this case and reviewing relevant literature, we summarize the clinical characteristics of contrast-induced encephalopathy presenting primarily with seizures, thereby providing clinical evidence to assist clinicians in the early diagnosis and optimal management of this condition.
X-linked Alport syndrome (XLAS) is a hereditary glomerular basement membrane (GBM) disease caused by COL4A5 mutations, leading to end-stage renal disease. With unclear pathogenesis and limited treatments, reliable animal models are urgently needed. In this study, the mutation K229X in COL4A5 detected in XLAS patients was introduced into mice model by CRISPR/Cas. The clinical manifestations and pathological changes in the K229X mice were characterized through urinary and serum tests, histopathology, immunofluorescence, and transmission electron microscopy. In K229X male mice, we observed significant hematuria and proteinuria, along with azotemia, and noted a marked decrease in the expression of COL4A5 at both the mRNA and protein levels within the kidneys. Pathological examination revealed glomerulosclerosis, increased mononuclear cells in the renal interstitium, interstitial fibrosis, and absence of α5 collagen IV, with histological abnormalities in the glomeruli, renal tubules, and interstitium progressing with age. Electron microscopy found irregular thickening of the GBM, accompanied by irregular layering. The phenotypic and pathological features of this mouse model are consistent with those observed in XLAS patients and other previously established mouse models. This K229X mouse model is of significant importance for exploring the pathogenic mechanisms of XLAS and researching potential therapeutic approaches.
Integrated computational-experimental platforms are promising for GPCR drug discovery, but systematically connecting high-resolution structural prediction with functional validation remains challenging. Here, we apply an integrated platform to discover a novel, potent antagonist targeting the P2Y14 receptor (P2Y14R), a key target in inflammatory diseases. Combining structure-based virtual screening, BPMD, all-atom molecular dynamics simulations, and cellular cAMP functional assays, our platform pinpointed Benfotiamine, an approved drug, as a nanomolar-potency P2Y14R antagonist (IC50 = 0.31 nM). Computational analyses indicated that Benfotiamine exerts a "conformational lock" to stabilize the inactive receptor and hinder Gi coupling. Subsequent CETSA and SPR experiments confirmed its direct binding to the target. Functionally, Benfotiamine exhibited therapeutic efficacy in murine models of dextran sulfate sodium-induced colitis and monosodium urate-induced gouty arthritis. Beyond presenting a promising repurposed anti-inflammatory agent, this study validates a modular and extensible computational-experimental integration platform that can be widely applied to expedite the discovery and mechanistic characterization of GPCR-targeted therapeutics.
Breast cancer molecular subtypes differ substantially in treatment selection, therapeutic response, and prognosis, making accurate subtype identification essential for individualized management. Current molecular subtyping mainly relies on immunohistochemical and molecular pathological analyses of biopsy or surgical specimens, which are invasive and may be affected by sampling bias, intratumoral heterogeneity, and testing turnaround time. This study aimed to evaluate the diagnostic performance of multimodal ultrasonography for noninvasive molecular subtyping of breast cancer. A total of 155 patients diagnosed with breast cancer were retrospectively enrolled. According to molecular classification, patients were categorized into the human epidermal growth factor receptor 2 (HER2)-overexpression subtype (n=32), triple-negative subtype (n=31), luminal A subtype (n=26), and luminal B subtype (n=66). Differences in multimodal ultrasound features among the various molecular subtypes were compared, and the predictive value of multimodal ultrasound parameters for molecular classification was analyzed. Higher maximum elastic modulus (Emax) and lesion and elasticity ratio between the lesion and surrounding normal tissue (Eratio) values were observed in the HER2-overexpression and triple-negative subtypes than in the luminal A and luminal B subtypes (P<0.05). Multivariate logistic regression analysis identified calcification status, Adler blood flow grading, vascularization index (VI), and Eratio as independent predictors of breast cancer molecular subtype (P<0.05). Receiver operating characteristic curve analysis showed that the combined multimodal ultrasonographic model demonstrated favorable diagnostic performance in identifying different molecular subtypes of breast cancer, with area under the curve (AUC) of 0.856 for HER2-overexpression, 0.973 for triple-negative, 0.900 for luminal A, and 0.811 for luminal B subtypes. The corresponding sensitivity/specificity values were 93.8%/71.5%, 96.8%/87.9%, 88.5%/82.2%, and 89.4%/66.3%, respectively. DeLong's test showed that the AUCs of the combined model were significantly higher than those of the individual ultrasound parameters for all four molecular subtypes (all P<0.05). The molecular subtypes of breast cancer exhibited distinct multimodal ultrasonographic characteristics. Multimodal ultrasonography showed potential value for noninvasive prediction of breast cancer molecular subtypes and may provide auxiliary information for individualized therapeutic decision-making.
[This corrects the article DOI: 10.3892/ol.2015.3893.].
Hepatocellular carcinoma (HCC) with tumor thrombus extending into the inferior vena cava (IVC) represents an advanced stage of disease and is associated with a poor prognosis. Surgical resection combined with thrombectomy remains a potentially curative option but carries substantial perioperative risk. Intraoperative transesophageal echocardiography (TEE) provides real-time imaging that may improve procedural safety and surgical precision. We report the case of a 49-year-old woman with advanced HCC complicated by tumor thrombus extending from the left portal vein and left hepatic vein into the IVC, with intermittent prolapse toward the right atrium. Preoperative assessment showed Child-Pugh class A liver function (score 6), an Eastern Cooperative Oncology Group (ECOG) performance status of 2, chronic hepatitis B infection without prior antiviral therapy, and preserved coagulation function. Imaging demonstrated cirrhosis and splenomegaly. The patient underwent left hemihepatectomy with IVC tumor thrombectomy under continuous intraoperative TEE guidance. TEE enabled dynamic assessment of thrombus mobility, guided repositioning of the thrombus from the right atrium into the IVC, informed the clamping strategy, and allowed immediate detection of acute bland thrombus formation after vascular repair. Prompt re-intervention was undertaken, and IVC patency was successfully restored. Histopathology confirmed moderately to poorly differentiated HCC with vascular invasion and tumor thrombus [pT4N0M0, American Joint Committee on Cancer (AJCC) 8th edition]. Postoperatively, the patient developed intrahepatic recurrence and received transcatheter arterial chemoembolization (TACE) and targeted therapy. At 12-month follow-up, no recurrence of IVC tumor thrombus was detected. This case highlights the value of anesthesiologist-led intraoperative TEE in complex oncovascular surgery. Continuous TEE guidance facilitated key vascular maneuvers, supported real-time intraoperative decision-making, and enabled early recognition of a potentially life-threatening complication. TEE should be considered an important intraoperative navigation tool in high-risk HCC thrombectomy.
Microplastics have been known to possess reproductive toxicity and can contribute to adverse pregnancy outcomes; however, their specific impact on the immune microenvironment at the maternal-fetal interface remains unclear. This study revealed that polystyrene microplastics (PS-MPs) accumulate extensively in macrophages at the maternal-fetal interface and promote their polarization toward a pro-inflammatory phenotype, ultimately leading to fetal growth restriction. Proteomic analysis indicated that microplastic exposure significantly enriched proteins associated with phenylalanine metabolism in macrophages. Further assays demonstrated a marked increase in intracellular phenylpyruvic acid levels, suggesting it as a key effector molecule mediating abnormal macrophage polarization in this process. Mechanistically, phenylpyruvic acid binds to acid ceramidase-1 and accelerates its degradation, which subsequently elevates ceramide levels and activates the MAPK/ATF2/COX2 signaling pathway. This cascade upregulates inflammatory factors, ultimately causing dysregulation of maternal-fetal interface macrophages and impaired fetal development. A phenylalanine-restricted diet significantly alleviated PS-MPs-induced aberrant macrophage polarization and fetal growth restriction. This study revealed the PS-MPs-induced adverse pregnancy outcomes through modulation of immune cell fate for the first time, providing a novel metabolic-immune perspective for understanding pregnancy complications associated with microplastics exposure.
Carbapenem-resistant Enterobacteriaceae (CRE) is the most common clinical pathogens. Investigating the antimicrobial resistance, hypervirulence and clinical characteristics of CRE isolated from children is helpful to guide for anti-infection treatments. Nonduplicated CRE clinical strains were isolated and mass spectrometry was applied to identify clinical isolated strains. VITEK 2 Compact system and Kirby-Bauer method were used to analyze the antimicrobial susceptibility. Besides, the drug resistance and hypervirulence associated genes were detected by polymerase chain reaction (PCR) and sequencing. A total of 281 non-duplicated CRE strains were identified in this study. Klebsiella pneumoniae (55.87%), Escherichia coli (36.65%) and Klebsiella aerogenes (3.20%) were the top 3 CRE strains. These strains showed high resistance to most of antimicrobial agents and carried carbapenemase genes, including bla OXA-232 (38.08%), bla KPC-2 (20.28%), bla OXA-1 (17.08%), bla NDM-5 (10.32%) and bla NDM-1 (8.90%). In addition, bla TEM-1 (98.93%), bla CTX-M-14 (88.61%) and bla SHV-11 (87.54%) were the prevalent extended-spectrum β-lactamase (ESBL) genes in these strains, while the detection rate of AmpC cephalosporinase genes were not high. Besides, 45 (28.66%) carbapenem-resistant Klebsiella pneumoniae (CRKP) strains carried hypervirulence associated genes iucA (24.84%), prmpA (17.83%), peg-344 (17.20%), and prmpA2 (9.55%). Among them, 22 (14.01%) CRKP strains were also identified as carbapenem-resistant and hypervirulent Klebsiella pneumoniae (CR-HVKP). What's worse, the patients infected with CR-HVKP had a worse prognosis overall. This study revealed the drug resistance, hypervirulence and epidemiology of CRE strains in pediatric patients in Suzhou of eastern China. Unfortunately, CR-HVKP strains with more several infection were also identified, which should be of great concern to clinicians.
This research aims to investigate how aberrantly expressed miR-25-3p and EZH2 regulate T cell activation in aplastic anemia (AA) patients and to explore the underlying mechanisms. The study enrolled 26 severe AA (SAA) patients and 22 healthy subjects. Quantitative reverse transcription polymerase chain reaction was used to detect the miR-25-3p, EZH2 and CD69 expression. After establishing an AA mouse model, cell viability, proteins expression levels and cytokines levels were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Western blot analysis, and enzyme-linked immunosorbent assay. A luciferase reporter assay was performed to verify the interaction between miR-25-3p and EZH2. Serum miR-25-3p expression was decreased in SAA patients (P < 0.001). Overexpression of miR-25-3p reduced cell viability and decreased CD69, interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) levels; conversely, inhibition of miR-25-3p exerted the opposite effect (P < 0.001). EZH2 was a downstream target gene of miR-25-3p. Overexpression of EZH2 partially reversed the decrease in cell viability and the inhibition of CD69, IFN-γ and TNF-α levels caused by miR-25-3p upregulation (P < 0.01). MiR-25-3p was downregulated in SAA patients and regulated CD4+ T cell activation and proliferation by targeting EZH2. These findings provide novel insights into potential therapeutic targets for AA.
Emerging evidence highlights that metabolic reprogramming profoundly shapes the tumor microenvironment and immune evasion in prostate cancer. However, the functional role and mechanisms of tryptophan metabolism in prostate cancer progression remain unclear. Through single-cell transcriptomic analysis, we identified one tumor cell subtype characterized by high expression of 3-hydroxyanthranilate 3,4-dioxygenase (HAAO) and enhanced kynurenine pathway activity. This subpopulation leads to the accumulation of quinolinic acid (QA), a metabolic intermediate that could activate the mevalonate (MVA) pathway. Mechanistically, QA directly binds to and stabilizes farnesyl diphosphate synthase (FDPS), a key MVA pathway enzyme, thereby enhancing cholesterol biosynthesis and fueling androgen receptor (AR)-driven transcriptional programs. This HAAO/QA-FDPS axis establishes a metabolic crosstalk that links tryptophan catabolism to lipid metabolism, sustaining prostate tumor progression. Furthermore, an integrated prognostic model incorporating this pathway signatures outperforms other clinical variables alone, and HAAO-high tumors exhibit heightened sensitivity to combined inhibition of the kynurenine and AR pathways. Our study unveils a novel metabolic vulnerability in prostate cancer and provides a mechanistic rationale for targeting the HAAO/QA-FDPS axis for therapy.
Sulfur-containing wastes are typical pollutants generated from oil and gas extraction, petroleum refining, and fossil fuel combustion, with annual global emissions reaching tens of millions of tons. Traditional treatment technologies rely on end-of-pipe control, suffering from high energy consumption, substantial carbon emissions, severe secondary pollution, and low resource utilization efficiency. Electrocatalytic conversion driven by renewable electricity enables the directional valorization of sulfur-containing pollutants under mild conditions, providing an alternative route for the green upgrading of sulfur resources. This review systematically summarizes the research progress in electrocatalytic valorization technologies for waste sulfur-containing species. Starting from the fundamental reaction principles, we elaborate the electrocatalytic conversion routes, reaction mechanisms, catalyst design, and system optimization strategies for four typical sulfur-containing substrates, including inorganic sulfides, sulfur oxides, organosulfur wastes, and metal sulfide minerals. It highlights the core technological innovations of direct interfacial electrocatalysis, redox mediator-mediated indirect electrocatalysis, and paired electrolysis. Moreover, the key challenges in anti-sulfur-passivation electrodes, reactor scale-up, reaction process design, and adaptation to complex industrial systems are outlined, and future perspectives are proposed. This review aims to provide theoretical and technical guidance for the green and low-carbon valorization of industrial sulfur-containing pollutants.
Magnetic resonance imaging (MRI) typically relies on metal-based contrast agents to enhance tissue visibility, but these agents raise safety concerns. Conjugated polymers present a promising organic alternative, generating T2 MRI contrast through the presence of unpaired organic electrons, known as polarons. However, the low polaron content limits the imaging capabilities of conjugated polymer-based MRI agents. This study explores strategies for effectively enhancing T2 MRI contrast in polypyrrole nanoparticles by increasing polaron content and optimizing interactions with water protons. Our results demonstrate that active polarons can be promoted by altering oxidative polymerization conditions. Furthermore, we observe a trade-off between polaron content and its interactions with water protons. This work investigates the underlying mechanisms by which structural parameters affect T2 MRI contrast, providing a clearer understanding of how to improve the imaging capabilities of conjugated polymer-based MRI agents.
Developing a high-performance and fully integrated photoelectrochemical (PEC) sensing platform for the on-site detection of antibiotic residues still remains challenging, as the key lies in constructing photoactive materials and integrated electrode arrays. Herein, we introduce highly conductive and hydrophilic Ti3C2Tx MXene to engineer the interfacial charge dynamics of bismuth sulfide (Ti3C2Tx/Bi2S3), addressing the rapid charge recombination and low conductivity in Bi2S3-based biosensors. This strategy not only facilitates electron extraction but also establishes a Schottky barrier suppresses electron backflow, thereby boosting photoelectric conversion efficiency for enhanced detection sensitivity. A fully integrated photoelectrode array is then fabricated by assembling Ti3C2Tx/Bi2S3 with carbon nanotubes via a template filtration method, yielding superior conductivity, stability, and reproducibility. This array features adjustable multichannel Ti3C2Tx/Bi2S3 disc working electrodes alongside integrated Ag/AgCl disc reference/counter electrodes. As a proof of concept, aptamer-functionalized sensing arrays achieve detection limits as low as 3.91 pM for chloramphenicol (CAP) and 4.91 pM for amoxicillin (AMX) in simultaneous assays, demonstrating robust selectivity in complex real samples. This work establishes a generalized strategy for translating interfacial charge regulation into high-performance, fully integrated PEC biosensing devices, advancing toward portable and multiplexed bioanalysis.
Whether metabolites enriched at early developmental stages affect cellular and organismal aging remains unclear. In this study, we comprehensively profiled the metabolic landscape of mouse oocytes in comparison to cleavage-stage embryos. Our analysis revealed that oocytes display accumulation of reductive metabolites that diminish following fertilization. Notably, we identified serotonin (5-hydroxytryptamine, 5-HT) as an oocyte-enriched metabolite with protective roles in aging. The underlying mechanisms operate through dual pathways: (i) in a canonical pathway serotonin acts via its receptor 5HTR1B to modulate mitochondrial function, and (ii) in a non-canonical pathway serotonin promotes serotonylation of HSP90β, which effectively reduces endoplasmic reticulum stress. Overall, our study demonstrates that oocyte-enriched metabolites including serotonin can alleviate aging-related cellular and systemic phenotypes, suggesting new avenues for anti-aging strategies.