搜索结果：Mitochondrial DNA. Part B, Resources

The mushroom Pleurocybella porrigens is widely distributed in temperate regions of the Northern Hemisphere. However, in the autumn of 2004, 59 food-poisoning incidents were reported in Japan, resulting in 17 deaths due to acute encephalopathy. In previous studies, we demonstrated that a lectin (PPL), pleurocybelline (PC), and pleurocybellaziridine (PA) from this mushroom caused neurotoxic effects in mice, and we also reported its nuclear genome (2013, 2026) and transcriptome data from fruiting bodies and mycelia (2024). Here we report the complete mitochondrial genome sequence of P. porrigens. The mitochondrial genome of P. porrigens is 63,054 bp in length and contains 18 protein-coding genes, 2 rRNA genes, and 25 tRNA genes. Phylogenetic analysis based on 14 conserved mitochondrial protein-coding genes recovered P. porrigens as a distinct and well-supported lineage within Agaricales. In the inferred topology, P. porrigens was positioned basal to the core Agaricales clade, internal to Clavaria fumosa and Hygrophorus russula, and clearly separated from Boletales outgroup taxa. This phylogenetic placement is consistent with recent phylogenomic frameworks recognizing Phyllotopsidaceae as an independent lineage within Agaricales. This study represents the first report of a complete mitochondrial genome from the genus Pleurocybella and provides a valuable reference for future comparative mitochondrial genomic studies within Agaricales.

The mitochondrial genome of C. laorentou was sequenced using Illumina in this study. The length of the circular mitochondrial genome is 56,088 bp, with a GC content of 30.26%. It encodes 44 genes, including 14 core protein coding genes (PCGs), 26 tRNA genes, 2 rRNA genes, 1 rps3 gene, and 1 free open reading frame (ORF). A phylogenetic tree was constructed based on a joint dataset of 14 mitochondrial core PCGs, which indicate that C. laorentou has the closest genetic relationship with Infundibulycybe hongyinpan and Leucopaxillus giganteus. This study provides the first mitogenome resource for the genus Catathelasma, offering crucial molecular data for its taxonomic placement and enriching fungal mitochondrial genome databases.

The genus Pseudolysimachion (Plantaginaceae) is currently considered a subgenus of Veronica, though its independent status is still debated. In Korea, Veronica subgenus Pseudolysimachion is diverse, including widespread Veronica longifolia (formerly Pseudolysimachion longifolium) and endemic Veronica pusanensis (formerly Pseudolysimachion pusanensis), both valuable for horticulture. Despite chloroplast genome utility in plant phylogenetics and breeding, complete chloroplast genome data for these key species were absent. To address this gap, we sequenced, assembled, and characterized the complete chloroplast genomes of V. longifolia and V. pusanensis. The complete chloroplast genomes of V. longifolia (152,237 bp) and V. pusanensis (152,250 bp) displayed typical quadripartite structures and encoded 133 genes (88 protein-coding, 37 tRNA, 8 rRNA). Phylogenetic analysis strongly supported the monophyly of the Veronica subgenus Pseudolysimachion clade, which is nested deeply within the genus Veronica. Our findings provide essential genomic resources that clarify phylogenetic relationships within the subgenus and contribute significantly to the broader taxonomic resolution of the tribe Veroniceae. These results firmly establish the taxonomic positions of the Korean endemic species, V. longifolia and V. pusanensis, and offer crucial genomic insights into speciation patterns and evolutionary trends within the genus, thereby supporting future plant breeding and germplasm utilization strategies.

Trirachys orientalis (Hope, 1841) is a long-horned beetle widely distributed in China with economic importance and potential medicinal value. In this study, we sequenced and assembled its complete mitochondrial genome. The complete mitogenome is 16,028 bp in length with a GC content of 29.60%, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. We further made the phylogenetic tree on the complete mitochondrial genomes of other 10 closely related species to show their phylogenic relationship. This genomic resource provides a fundamental baseline for future species identification, phylogenetic resolution, and the exploration of its potential utility as a biological resource.

Elachiptera decipiens is cereal pest distributed in North America, Europe, and China. Its complete mitochondrial genome from Xinjiang, China was sequenced using next-generation sequencing technique. The 18,344 bp mitogenome contains 13 protein-coding gene sequences (PCGs), 22 tRNAs, and 2 rRNAs. Phylogenetic analysis based on 13 PCG sequences at family level and COI gene sequences at subfamily level. Phylogenetic analysis revealed that E. decipiens is closely related to E. insignis. The complete mitogenome of E. decipiens would help understand Chloropidae evolution and develop rapid identification methods based on mitochondrial genes.

The Webb's tufted-tailed rat (Eliurus webbi) and the lesser tufted-tailed rat (Eliurus minor) are nocturnal and primarily arboreal rodents native to Madagascar, classified in the subfamily Nesomyinae. After species confirmation using cytochrome b, we assembled the first complete mitogenomes of E. webbi (n = 7) and E. minor (n = 3) from southeastern Madagascar. These mitochondrial genomes range from 16,310 to 16,313 nt for E. webbi and 16,296 to 16,302 nt for E. minor. These are the first assemblies of mitogenomes from Nesomyinae. This report enhances the evolutionary study and identification of Malagasy endemic rodents, and will support biodiversity research and wildlife conservation efforts.

The snowy sheathbill (Chionis albus (Gmelin, 1789)) is a coastal Antarctic bird that breeds on Antarctic and sub-Antarctic islands. In this study, we report the first complete mitochondrial genome sequence of C. albus. The mitogenome is circular, 19,116 bp in length, and contains 13 protein-coding genes (PCGs), including an ND3 gene with an extra nucleotide, 22 transfer RNAs (tRNAs), and two ribosomal RNAs (rRNAs). Notably, a duplicated region of ∼2.3 kb includes ND6 and two tRNAs. The overall nucleotide composition is 30.29% A, 30.37% C, 14.56% G, and 24.78% T, with a GC content of 44.93%. Phylogenetic analysis based on all 13 PCGs placed C. albus firmly within Charadriiformes and clustered with C. minor and, more distantly, with Burhinus bistriatus. This newly characterized mitogenome provides valuable resources for future studies on the phylogeny, molecular evolution, and biogeography of Charadriiformes and the family Chionidae.

Tetraselmis marina (Cienkowski) R.E.Norris, Hori & Chihara 1980, a marine green microalga, holds considerable value in aquaculture due to its well-rounded nutritional profile. Gaining insights into the mitochondrial genome of this species is crucial for advancing studies on its phylogeny and evolutionary relationships. In the present research, we for the first time characterized the complete mitochondrial genome of T. marina. This genome forms a circular structure with a total length of 51,196 base pairs (bp), encompassing 34 protein-coding genes, 24 transfer RNA (tRNA) genes, and 3 ribosomal RNA (rRNA) genes. Nucleotide composition analysis showed a relatively balanced distribution: adenine (A) accounted for 32.9%, thymine (T) for 33.8%, guanine (G) for 16.5%, and cytosine (C) for 16.8%. Phylogenetic investigations demonstrated that T. marina is classified within the Chlorodendraceae family and shares the closest evolutionary affinity with Tetraselmis sp. CCMP 881.

Microplitis varicolor (Hymenoptera: Braconidae) is a parasitoid wasp species with potential for biological control of lepidopteran pests. In this study, the complete mitochondrial genome of M. varicolor was sequenced and characterized. The mitogenome is 15,580 bp in length and exhibits a strong AT bias (86.82%). It contains 37 mitochondrial genes, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a control region. Phylogenetic analyses support the monophyly of Braconidae and place M. varicolor firmly within the genus Microplitis. The genome exhibits largely conserved organization with several tRNA rearrangements, providing a valuable resource for phylogenetic and evolutionary studies of Braconidae.

Trichaptum biforme, a globally recognized white-rot Basidiomycete, is a member of the Hymenochaetales order. This research focused on a specimen of T. biforme MES02, which was obtained from the Qinling Mountain range in China, specifically from Zhashui County, Shangluo City, Shaanxi Province. Employing next-generation sequencing (NGS) technology, the whole mitochondrial genome of T. biforme MES02 was elucidated. This genome encompasses 15 protein-coding genes (PCGs), 24 transfer RNAs (tRNAs), and two ribosomal RNAs, spanning a total of 55,598 base pairs (bp). The nucleotide composition was determined to be A (31.80%), C (16.94%), G (18.30%), and T (32.96%), with a GC content of 35.24%. Furthermore, a phylogenetic analysis was conducted, constructing a tree from 27 mitochondrial genomes. This study presents the first insight into the phylogenetic interrelations of T. biforme with its alld Basidiomycetes, providing a foundational framework for further evolutionary and taxonomic inquiries within this fungal group.

The complete mitochondrial genome of Bionychiurus tamilensis Thunnisa & Sumithra, 2021 was sequenced, assembled, and annotated. The mitochondrial genome of B. tamilensis is 14,937 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. The overall nucleotide composition was 35.3% A, 15.8% C, 9.8% G, and 39.6% T, indicating an obvious A + T bias (74.9%). B. tamilensis was closely clustered with the following species of Onychiuridae: Allonychiurus kimi, Tetrodontophora bielanensis, Onychiurus orientalis, and Orthonychiurus folsomi.

Leptoglossus occidentalis (Heidemann, 1910) (Hemiptera: Coreidae) is an invasive species that has invaded China. This study reports the first sequencing and assembly of its complete mitochondrial genome. The results show that the mitochondrial genome of L. occidentalis contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a non-coding control region, with a total length of 15752 bp and an A + T content of 72.9%. Based on phylogenetic analyses, L. occidentalis and L. membranaceus (Fabricius, 1781) are sister species. Additionally, in Tianjin, Shaanxi, and Guangdong Provinces, new distribution records of this species were documented in this study.

We report the complete mitochondrial genome (mitogenome) of Pryeria sinica (Lepidoptera: Zygaenidae). The mitochondrial gene arrangement conforms to be conserved in Zygaenidae, including P. sinica, and is characterized by an inverted translocation of the trnE-trnS1 block between trnN and trnF (underlined gene names indicate counter-clockwise transcription). Phylogenetic analyses strongly supported each subfamily monophyly with the highest nodal supports. Within Zygaenidae, Chalcosiinae and Procridinae formed a sister group, with the highest nodal supports. Within Zygaeninae, P. sinica was identified as a sister group to Zygaena species, with the highest nodal supports.

Mitochondrial genomes of the annelid family Serpulidae are characterized by a high variation in nucleotide divergence and gene arrangements. However, mitogenomic data from the genus Serpula remain unavailable. We report the complete mitochondrial genome of Serpula uschakovi from the Sea of Japan. The circular mitogenome is 20,628 bp in length, containing 13 protein-coding, two rRNA, 22 tRNA genes, and nine large non-coding regions. Its gene order exhibits extensive rearrangements when compared to the putative Pleistoannelida pattern yet retains a highly conserved 10 PCGs block shared with the genus Hydroides. Phylogenetic analysis placed S. uschakovi as the sister group to Hydroides.

Schistura rara, a small stream-dwelling fish of the family Nemacheilidae, is endemic to headwater streams of the Beijiang River in Shaoguan, China. Here, we report the first complete mitochondrial genome (mitogenome) of S. rara, which is 16,563 bp in length and contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a single non-coding control region (D-loop). The mitogenome has a GC content of 42.8% and shows a clear bias toward A/C-ending codons in PCGs. Maximum-likelihood (ML) phylogenetic analyses based on complete mitogenome sequences indicate that Schistura is non-monophyletic. Within this framework, S. rara is recovered as the sister species to S. incerta and, together with one lineage of S. fasciolata, forms a well-supported clade. These three species are the only Schistura taxa recorded from the upper Beijiang River. The newly generated mitogenomic data provide an important genetic resource for clarifying the phylogenetic position and taxonomic status of S. rara within Nemacheilidae.

Umbelopsis nana is a saprotrophic filamentous fungus that plays a role in nutrient cycling in forest ecosystems. We determined the complete mitochondrial genome sequence of U. nana strain THIF13 (NBRC 117090), which is a circular molecule of 39,484 bp with a GC content of 32.7%. The mitogenome encodes 45 genes, including 20 protein-coding genes, 23 tRNA genes, and two rRNA genes. Phylogenetic analysis based on 13 Mucoromycota species and two outgroup taxa placed U. nana as a sister lineage to the Mucorales clade within Mucoromycotina. This study represents the first report of a complete mitochondrial genome sequence for the genus Umbelopsis.

Ophryotrocha xiamenensis is an appropriate model for investigating regeneration and evolution. The complete mitochondrial genome is 16,111 bp in length and consists of 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and a noncoding region. Phylogenetic analysis based on concatenated sequences of all 13 PCGs using the maximum-likelihood and Bayesian methods, placed O. xiamenensis within the 'labronica' clade and the order of gene arrangement was the same as that of O. japonica. This study contributes to the development of genetic resources and advances the understanding of phylogenetic resolution within the genus Ophryotrocha.

The complete mitochondrial genome of Synandwakia multitentaculata, a marine protected species endemic to the western coast of Korea, was sequenced and analyzed. The circular mitogenome is 18,350 bp with AT-rich composition (G + C content: 34.3%) and contains 17 genes: 13 protein-coding genes (PCGs), two ribosomal RNAs , and two tRNAs. All PCGs are encoded on the heavy strand and initiate with ATG. The mitogenome shows characteristic Actiniaria split-gene architecture in COX1 and ND5, with ND1 and ND3 located within the ND5 intron. Phylogenetic analysis of 13 PCGs placed S. multitentaculata with Diadumene lineata within Metridioidea, providing the first mitochondrial genomic resource for Synandwakia and the family Andvakiidae.

Here, we report the complete mitochondrial genome of Cossura pygodactylata Jones 1956 (Annelida, Sedentaria, Cossuridae) (20,713 bp) comprised of two ribosomal RNAs, the ubiquitous set of 13 protein-coding genes (PCGs), 24 tRNAs, and a long AT-rich non-coding region. The order of PCGs and rRNAs is the same as in the previously reported genome of Cossura aciculata Wu & Chen 1977, but the order of tRNAs is different. Phylogenetic analysis of PCGs confirmed that Cossuridae is monophyletic and represents the sister group to Cirratulidae within Cirratuliformia.