U.S. Mexican-born Hispanic/Latino households are at a high risk of experiencing food insecurity and its poor dietary and health consequences but may have attributes that can be leveraged to promote food security and healthy dietary quality. To gain more in-depth understanding and capacity-oriented data to inform programming, we sought to elucidate potential food provisioning-related factors protecting low-income U.S. Mexican-born households with young children from food insecurity. We conducted a community-based qualitative study, guided by Life Course Perspective and Ecological Systems Theory, in two New York State counties. Low-income Mexican-born mothers (n = 27) of young children (≤5 years) with ≤10 years in the U.S. completed two semi-structured interviews, including a participant-driven photo elicitation interview, and responded to the 18-item Household Food Security Survey Module. We conducted thematic content analyses to identify emergent themes. We identified four themes related to important attributes and behaviors of participants, which appeared to protect their households from food insecurity: resourcefulness and creativity with food provisioning, valuing new knowledge and skill-building opportunities, reflectiveness on poverty and food insecurity experiences, and the ability to establish beneficial food-provisioning routines, with the latter appearing to differentiate food-secure and food-insecure households. These attributes and behaviors facilitated management of economic resources while also meeting cultural ideals and family preferences. Low-income Mexican-born households appear to have human and cultural capital (i.e., assets) that may serve to be protective. Improved policies and programming are necessary to leverage these capacities to promote food security and healthier dietary intake.
This cross-sectional study aimed to assess the relationship between food insecurity, consumption of school meals, and eating behavior of students enrolled in the campuses of a federal educational institution in São Paulo. The main variables in the study were the level of food and nutritional (in)security, and the independent variables were sociodemographic factors, consumption of school meals, and eating behavior. Pearson's chi-square test and multinomial regression were used to assess the relationship between the level of nutritional food (in)security and the variables. A total of 702 students participated in the study, 49.86 per cent of whom lived in food-insecure households. Daily consumption of school meals was associated with food insecurity, as did not eat breakfast or foods that mark a healthy diet. Thus, the supply and consumption of school meals, as well as sociodemographic factors, showed a relationship with food security, and it is therefore crucial to understand the situation of food and nutritional security among students to better target institutional policies and programs. Estudo transversal com o objetivo de analisar a relação entre a insegurança alimentar, o consumo da alimentação escolar e o comportamento alimentar dos estudantes matriculados no ensino médio e técnico de todos os campi de uma instituição de ensino federal de São Paulo. A principal variável do estudo foi o nível de (in)segurança alimentar e nutricional, e as variáveis independentes foram sociodemográficas, consumo da alimentação escolar e comportamento alimentar. Para avaliar a relação entre o nível de (in)segurança alimentar nutricional e as variáveis foram realizados o teste qui-quadrado de Pearson e regressão multinomial. Participaram do estudo 702 estudantes, sendo que 49,86% viviam em domicílios com insegurança alimentar. O consumo diário da alimentação escolar se relacionou com a insegurança alimentar, bem como não consumir café da manhã e alimentos marcadores de uma alimentação saudável. A oferta e o consumo da alimentação escolar, assim como fatores sociodemográficos, demonstraram relação com a segurança alimentar, sendo, portanto, crucial o conhecimento da situação da segurança alimentar e nutricional entre os estudantes atendidos para melhor direcionamento das políticas e programas institucionais. Estudio transversal con el objetivo de analizar la relación entre la inseguridad alimentaria, el consumo de la alimentación escolar y el comportamiento alimentario de estudiantes de la educación secundaria y técnica de una institución de enseñanza federal de São Paulo. La variable principal del estudio fue el nivel de (in)seguridad alimentaria y nutricional, y las variables independientes fueron sociodemográficas, consumo de la alimentación escolar y comportamiento alimentario. Para evaluar la relación entre el nivel de (in)seguridad alimentaria nutricional y las variables, se realizó el Chi-cuadrado de Pearson y regresión multinomial. Participaron 702 estudiantes, de los cuales el 49,86% vivía en hogares con inseguridad alimentaria. El consumo diario de la alimentación escolar se relacionó con la inseguridad alimentaria, así como el no consumir desayuno y alimentos que son marcadores de una dieta saludable. La oferta y consumo de comidas escolares, así como los factores sociodemográficos, demostraron una relación con la seguridad alimentaria, siendo crucial conocer la situación de seguridad alimentaria y nutricional de los estudiantes atendidos, para orientar mejor las políticas y programas institucionales.
Climate change, rice viruses, and viruses vector pests are major threats of global rice production. The future climate could create more suitable habitats for insect vectors that transmit rice viruses. Here we leveraged large-scale data to examine present and future (2041-2060) suitable habitats of six rice viruses that were transmitted by 10 insect vectors. We associated these habitat predictions with rice cultivation areas to assess potential climate change impacts on rice productions. Under the future climate, the suitable habitats of rice virus vector pests are expected to expand: the habitat area is projected to increase by 34.97% under the local dispersal scenario and up to >230% under the full dispersal scenario in 2041-2060. Among the six major rice viruses, rice dwarf virus and rice stripe virus pose the greatest threats in India and China, respectively, under the future climate. Emerging risk regions were identified in North America, South America, and Africa. More than 70% of rice cultivation areas in China and India fall into potential risk areas of two or more insect vectors. Under both the local and full dispersal scenarios, these infested areas are projected to increase to approximately 80% during 2041-2060. In regions facing poleward expansion or potential long-distance invasions, our findings underscore the need for adaptive strategies, including strengthened international quarantine measures, enhanced pest monitoring networks, and early warning systems, to mitigate the potential impacts of climate change on rice production. © 2026 Society of Chemical Industry.
There are limited data on food insecurity among college students from different marginalized backgrounds, both in Vermont and other predominantly White spaces, and what their institutions could do to improve food security and other basic needs. Thus, the aims of this study were to 1) examine food insecurity prevalence and its correlates among underrepresented college students, 2) assess their reasons for using campus food pantries and strategies for improving access to culturally appropriate foods on campus, and 3) determine their perspectives regarding how their institutions can address their basic needs. Using a mixed methods cross-sectional research design, a convenience sample of full-time students or trainees ages 18 and older enrolled at four institutions of higher education in Vermont, United States, were recruited through flyers, course announcements, newsletters, listservs, and social media posts. An online survey provided estimates of food insecurity, on-campus food pantry use, and participant perspectives on institutional strategies to address basic needs through open-ended questions. Food insecurity was assessed by the 10-item United States Department of Agriculture food security survey module. Descriptive statistics assessed frequencies and percentages along with means and standard deviations. Binary logistic regression was conducted to estimate the odds of food insecurity. Inductive coding was used to analyze qualitative data and identify emergent themes. Around 40% of all participants experienced food insecurity in the past year, with higher odds observed in students aged ≥ 30, Pell Grant recipients, first-generation students, Black students, and those who used a food pantry. The most cited reason for using a campus food pantry was ease of getting to the pantry while lack of awareness was the most reported barrier. Over 50% of participants recommended gathering feedback from students about their preferences to improve access to culturally appropriate foods. Common themes from qualitative analyses included: food access support, safe and affordable housing access, mental health and healthcare access, financial aid access, reliable and affordable transportation access, non-financial academic support, and general support services. Institutions should prioritize food assistance programs targeting college students and incorporate mental health care, housing support, and financial aid for comprehensive safety nets.
Food insecurity (FI) is an unmet health-related social need that affects child health. Our pediatric clinic partnered with a community food pantry, New York Common Pantry (NYCP), to provide emergency food onsite and facilitate referrals. We examined the impact of completing these referrals on household FI over 12 months. We conducted a prospective study in a general pediatric practice that screens for FI using the Hunger Vital Sign™ tool. Families with children ages 0-10 years who spoke English or Spanish and experienced FI were enrolled. Caregivers completed the 18-item USDA Household Food Security Survey (HFSS), which scores FI from 0 (high food security) to 18 (very low food security) at baseline, 3 and 12 months, and self-reported pantry enrollment status at follow-ups. We examined the impact of pantry enrollment on food security with an adjusted mixed-effects linear regression, using HFSS score as a continuous variable. We enrolled 125 families; 66% children identified as Hispanic and 26% non-Hispanic Black; 93% had Medicaid insurance. Median baseline HFSS score was 4.0 (2.0, 6.5), considered "low food security." At baseline, 84 (67%) caregivers accepted NYCP referral; an additional 20 accepted referral over the study period. In adjusted mixed-effects models, households enrolled with NYCP at both 3 and 12 months had significantly lower (improved) HFSS scores at both 3 months (estimate -3.40, p=0.016) and 12 months (estimate -3.11, p=0.026) compared to those not enrolled. A community-academic partnership promoted enrollment in a food pantry and improved FI. Future research is needed to identify strategies to improve referral completion among families referred to food pantries by a pediatrician.
Food insecurity and energy insecurity are critical global health issues, with implications for dietary diversity and infant feeding practices. This study evaluated the effects of an intervention for a liquefied petroleum gas (LPG) cook-stove and for free fuel on food security and dietary diversity among women and infants in four diverse settings. The Household Air Pollution Intervention Network (HAPIN) study is a randomized controlled trial that enrolled 800 pregnant women each in Guatemala, Peru, India, and Rwanda. Participants (N=3195) were randomly assigned to intervention or control groups, with intervention households receiving an LPG cook-stove and free fuel for 18 months. We measured household food insecurity using the Food Insecurity Experience Scale and dietary diversity for women and infants using the Minimum Dietary Diversity - Women and WHO infant and young child feeding questionnaires, respectively. We conducted an intention-to-treat analysis to evaluate the effect of the intervention on each outcome. At baseline, 27.4% of households were mildly food insecure, 15.6% were moderately or severely food insecure, and 26.8% of women achieved minimum dietary diversity. At end-line, the intervention significantly reduced moderate or severe food insecurity (RR: 0.75, 95% CI: 0.58-0.97) and improved dietary diversity among our analytic sample of 2868 women (RR: 1.16, 95% CI: 1.01-1.33) and 2836 infants aged 12 months (RR: 1.07, 95% CI: 1.002-1.15). Our findings suggest that interventions that increase energy security can be nutrition-sensitive and contribute to improved food and nutrition security. The trial was registered on clinicaltrials.gov (Identifier NCT029446282) on October 17, 2016.
Food market accessibility is a critical yet underexplored dimension of food systems, particularly in low- and middle-income countries. In this paper, we present a continent-wide assessment of spatial food market accessibility in Africa, integrating open geospatial data from OpenStreetMap and the World Food Programme. We compare three complementary metrics: travel time to the nearest market, market availability within a 30-minute threshold, and an entropy-based measure of spatial distribution, to quantify accessibility across diverse settings. We find pronounced disparities in accessibility: rural and economically disadvantaged populations face substantially longer travel times and reduced market availability, with some areas requiring several hours of travel. These accessibility patterns align with socioeconomic stratification, as measured by the Relative Wealth Index, and moderately correlate with food insecurity levels, assessed using the Integrated Food Security Phase Classification. Overall, results suggest that access to food markets reflects broader geographic and economic inequalities and plays a relevant role in shaping food security outcomes. Despite limitations related to incomplete and spatially heterogeneous market data coverage, this framework provides a scalable, data-driven approach for identifying relative structural market accessibility gaps, supporting equitable infrastructure planning and spatially informed food security analyses across diverse African contexts.
Food literacy is an emerging research area and focus of health promotion initiatives to improve nutrition outcomes. Food literacy is contextual, and definitions differ across specific populations or settings. Food literacy as a concept has not been well studied in Pacific Islander populations. The aim of this scoping review was to identify how food literacy is characterized and measured in the Pacific Islands context and describe food literacy health promotion initiatives that may inform policy, practice, and research. The research questions were as follows: (i) How has food literacy been defined, described, and measured in Pacific Islands countries and territories populations? (ii) What health promotion initiatives have been implemented to develop and improve food literacy in these populations? A scoping review using a published protocol was conducted to identify grey and empirical evidence that broadly defined, described, measured, or explored food literacy and/or its components within Melanesia, Polynesia, and Micronesia subregions, within the previous 30 years. The search using three academic databases and relevant websites identified 44 sources representing 16 Pacific Islands countries and territories. Of these, four sources used the term 'food literacy'. All other sources measured or promoted a food literacy knowledge, skill or behaviour, or a skill without directly naming it, highlighting the unique sociocultural elements influencing food literacy throughout this region. Understanding food literacy from this sociocultural context can help inform public health policy and health promotion practice, leading to more effective initiatives that reflect local values and practices and support local food systems.
Food and nutrition insecurity in high‑income countries is increasingly persistent, driven by intersecting economic, social and environmental disruptions. In Australia, acute shocks such as the COVID‑19 pandemic, floods and bushfires, alongside chronic pressures including rising food prices, housing stress and concentrated corporate power, have exposed structural weaknesses in food access, governance and system resilience. This review examines how community‑led responses to food and nutrition insecurity function during disruption, whether they buffer short-term hardship or contribute to adaptive capacity and redistribution of agency. Guided by the six‑pillar food security framework and a socio-ecological model, responses are examined across household, community, organisational and governance levels. A continuum of responses is identified, ranging from downstream emergency food relief that buffers immediate hardship, through to community and organisational food infrastructure that strengthens local resilience, and governance‑level responses with greater transformative potential. Drawing on this synthesis, we propose the SEEDS (Socio-Ecological Enablers of Dietary Security) Model, which conceptualises how food system responses across socio-ecological levels and over time can progress from buffering (acute) to adaptation (medium‑term), and ultimately transformation (long‑term). Central to this framework is a shift in decision‑making power, accountability and participation. While many initiatives improve food access and short‑term stability during crises, the greatest potential for transformation lies where responses are embedded within governance structures, enable meaningful community participation, and influence policy, procurement and resource allocation. Implications for public health nutrition practice include expanded roles in systems leadership, cross‑sector governance and advocacy for upstream policy reform.
To compare the prevalence of food insecurity (FI) in adults with spinal cord injury (SCI) versus a matched control cohort without self-reported disability, identify demographic and socioeconomic correlates of food insecurity, and examine the association between FI and body mass index (BMI). Cross-sectional analysis. U.S. nationwide cohort from the All of Us Research Program. A cohort of 751 adults with SCI and a 1:3 age-, sex-, race-, and ethnicity-matched control cohort. Not applicable. FI, BMI. Overall, 19.0% of participants with SCI (n=143) experienced FI, which was higher than the prevalence observed in the control cohort (15.6%, p<0.05). In univariate analyses, risks of FI were elevated among participants who were Black or Hispanic, women, younger, unmarried, unemployed, had lower educational attainment, and lower income (all p<0.05). In multivariable logistic regression, higher income, being retired, and education beyond high school were associated with lower odds of FI, whereas race, ethnicity, sex, and marital status were no longer significant. In BMI analyses, FI was associated with higher BMI in unadjusted models, but this association attenuated after covariate adjustment. Exploratory analyses suggested effect modification by race: among White participants, FI was associated with higher BMI (+1.73kg/m²; p<0.05), whereas among Black participants, FI was associated with lower BMI (-3.22kg/m²; pinteraction=0.02). Sensitivity analyses using BMI≥25 or ≥30kg/m² yielded consistent racial-specific patterns. These findings highlight food insecurity as an important social determinant of health in SCI, shaped largely by socioeconomic conditions rather than demographic and injury characteristics. The association between FI and weight status in SCI may differ across racial groups, warranting further investigation into the pathways through which material hardship influences health in this population.
Microbial interactions form food webs and are crucial for regulating food web stability. A structurally stable food web is a prerequisite for aquatic ecosystem functioning. Dam construction and reservoir operation significantly alter environmental conditions, in which hydrodynamic, trophic, and vertical variations affect all trophic levels of the hierarchy via top-down and bottom-up forces, with profound effects on microbial food web structure and stability. However, little is known about which external environmental drivers primarily influence the shaping of microbial food web structure, and how phytoplankton-heterotrophic bacteria interactions affect the stability of the entire microbial food web in river-reservoir systems. Here we explored multi-trophic interactions among phytoplankton, heterotrophic bacteria, and protists along trophic, longitudinal (hydrodynamic), and vertical gradients in five reservoirs of the upper Yangtze River. Hydrodynamic conditions explained more variation in microbial food web stability than trophic and vertical conditions. Food web stability declined along trophic, longitudinal, and vertical gradients, accompanied by reductions in α-diversity, K-strategist bacteria abundance, and food web complexity, together with an increase in the proportion of positive phytoplankton-heterotrophic bacteria associations. Potential phytoplankton-heterotrophic bacteria mutualism was intensified by an increase in trophic state, a reduction in water flow velocity, and a shift from bottom to upper layers, which had an adverse effect on microbial food web stability by reducing predation pressure. Our findings highlight the importance of hydrodynamic variations in shaping microbial food web structure and underscore the pivotal role of phytoplankton-heterotrophic bacteria interactions in affecting food web structural stability, providing critical insights for river-reservoir system management.
Regular monitoring of nutritional indicators serves as an early warning system for policymakers and is essential for the effective governance of food security. This study presents the first 18-year trend analysis of changes in Iranian household food purchases, with a specific focus on inequalities across income deciles and between urban and rural areas in Iran. Data from the Statistical Center of Iran (2005-2022) were analyzed. Six major food groups, calories, and macronutrients derived from household purchases were examined. Linear regression, with year as a continuous predictor, was performed using SPSS (p < 0.05 considered significant). Data processing and figure generation were conducted using Microsoft Access 2016 and Excel 2016, respectively. Over the 18-year study period, per capita purchases of all major food groups declined in both urban and rural areas. Urban-rural disparities in household food purchases, as well as the associated estimated calorie and macronutrient quantities, narrowed. Furthermore, although higher-income households consistently purchased more of most food groups (except cereals) than lower-income households, these income-based inequalities also diminished over time. Although nutritional inequalities in Iran have decreased in recent years, the simultaneous decline in overall household food purchases calls for more rigorous research on the subject. Analyzing long-term trends in household food purchase-based nutritional indicators is a critical scientific and policy priority for evaluating programs and designing evidence-based policies.
Background: The Veggie Van (VV) model is a mobile produce market intervention previously shown to increase fruit and vegetable (F&V) consumption among lower-income individuals. Although the model was recently evaluated in a multi-state effectiveness trial, preliminary findings suggested challenges to implementing the model with fidelity. This study examined whether variation in implementation fidelity was associated with participant-level dietary, food security, and market utilization outcomes. Methods: This study involved a secondary analysis of participant outcome data from a longitudinal RCT conducted with 9 implementing organizations operating 17 mobile market sites (n=699 participants). Previously collected participant data included baseline and 12-month measures of F&V consumption from 24-hour dietary recalls and self-reported surveys, as well as food security assessed using the USDA 10-item Adult Food Security Survey Module. Participant outcome data were aggregated at the site level and linked with organizational implementation data collected throughout the intervention period. Implementing organizations completed monthly process measures surveys assessing adherence to core VV model components; responses were scored and aggregated into site-level implementation fidelity scores. Correlational analyses and generalized linear models examined associations between implementation fidelity and changes in F&V consumption, food security, and market utilization outcomes over 12 months. Results: The mean implementation fidelity score across sites was 40.6 out of 55 possible points. Higher overall fidelity was positively associated with greater increases in mean F&V consumption over 12 months (Pearson correlation coefficient [PCC]=0.56, p=0.02). Fidelity to the nutrition education component demonstrated a particularly strong association with change in F&V consumption (PCC=0.81, p<0.0001). These associations remained significant in regression analyses. In addition, sites operating markets for ≥10 months annually had 76% greater odds of participants shopping at the mobile market at least once during the intervention period (p=0.04). No significant associations were observed between implementation fidelity and changes in food security. Conclusions: Greater fidelity to the VV model, particularly implementation of nutrition education components, was associated with improved dietary outcomes in this secondary analysis of participant-level RCT data. Findings highlight the importance of evaluating implementation alongside effectiveness outcomes in community-based nutrition interventions and suggest that tailored implementation support strategies may strengthen delivery of intervention components most strongly associated with dietary change. Trial registration: The larger randomized controlled trial, the Veggie Van study, was registered at https://clinicaltrials.gov/ on January 29, 2020 (NCT04246593).
Livestock-based livelihoods underpin food security, dietary quality, and resilience in African arid and semi-arid lands (ASALs), yet the mechanisms linking livestock systems, food environments, and human nutrition under climate variability remain fragmented. This PRISMA-based systematic review synthesizes evidence on the nutritional ecology of pastoralist and agro-pastoralist communities in Sub-Saharan Africa, focusing on livestock-mediated nutrition pathways shaped by ecological and socioeconomic change. Literature published between 2006 and 2026 was systematically identified from PubMed, Scopus, AJOL, CABI, and Google Scholar, yielding 60 studies for qualitative synthesis. Nutrition outcomes assessed across the reviewed studies included Household Dietary Diversity Score (HDDS), child wasting and stunting, milk intake, micronutrient adequacy, and food security indicators. The evidence shows that livestock support household nutrition through interconnected biological and economic pathways, including direct consumption of animal-source foods, particularly milk, income generation for food purchases, and buffering against seasonal and climate-related shocks. Larger and more diversified herds are generally associated with higher dietary diversity and improved child nutrition, but benefits are strongly mediated by mobility, market access, gendered control of livestock income, and environmental sustainability. Seasonal dynamics drive marked nutritional variation, with wet seasons supporting improved dietary quality and dry seasons linked to heightened food insecurity and child wasting. Constraints on mobility, sedentarization, market disruptions, and environmental degradation further amplify nutrition risks. The evidence base is dominated by cross-sectional study designs, limiting causal inference and constraining understanding of how livestock ownership, environmental conditions, and nutrition outcomes co-evolve across seasons and shocks. The review identifies key evidence gaps, including limited longitudinal studies, underrepresentation of urbanizing pastoralists, and minimal attention to youth nutrition and livelihood transitions. Drawing on a One Health and food-systems framework, addressing these gaps requires integrated nutrition-livestock surveillance and multisectoral policies to advance climate-resilient, nutrition-sensitive livestock development and support progress toward Sustainable Development Goals related to zero hunger, good health and well-being, and climate action in African drylands.
Ensuring high-quality care in assisted living (AL) is a growing priority, yet little is known about factors shaping resident-reported quality of life (QoL). In the United States, Minnesota recently implemented statewide measurement and public reporting of AL resident-reported QoL. Leveraging these unique data, we evaluated resident- and facility-level factors associated with 7 validated QoL domain scores: staff, environment, food, engagement, autonomy, culture, and security. Cross-sectional survey. Eleven thousand six hundred eighty-four AL residents in facilities with more than 5 beds, who participated in the 2024 statewide QoL survey (90% aged ≥65 years, 66% female, and 84% White). We identified resident- and facility-level correlates of AL resident-reported QoL in 7 domains, using multivariable linear mixed regression with facility-level random intercept to account for facility-level clustering. Mean domain-specific QoL scores ranged from 1.6 to 1.9 out of 2, with food and engagement rated lowest and environment highest. Older residents reported significantly higher scores across most QoL domains (β = 0.02-0.08; P < .05). Females rated food and security significantly lower (β = -0.04 and -0.02; P < .05), whereas American Indian/Alaskan Native residents reported significantly lower scores in culture, security, staff, and environment (β = -0.05 to -0.10; P < .05). Smaller facilities (<25 beds) had significantly higher scores in food, staff, and autonomy (β = 0.02-0.07; P < .05). Facilities with dementia care unit licensure had significantly higher food scores but significantly lower autonomy, staff, and security scores (β = - 0.02 to -0.03; P < .05). Our findings indicate sociodemographic differences in AL residents' QoL, with lower QoL among vulnerable subgroups. Patterns in resident-level and facility-level correlates of residents' QoL scores differ by QoL domain, underscoring the need for targeted interventions and policy efforts to enhance resident well-being in AL.
To realize the low-cost preparation of Nitrogen-doped carbon quantum dots (N-CQDs) for high-sensitivity Hg2+ fluorescence detection and their application in food sample analysis, this study used malonic acid as the carbon source and 2,3-diaminopyridine as the nitrogen source to prepare N-CQDs via a one-step microwave-assisted heating method by optimizing the synthesis conditions. The surface functional groups, molecular structure, optical properties, and Hg2+ response characteristics of the N-CQDs were investigated in detail. Finally, the N-CQDs were applied for Hg2+ detection in typical real samples (tap water, rice, and grass shrimp). This study provides new insights and methods for clarifying the structure-activity relationship between N-CQDs and Hg2+ response, and constructing a rapid and accurate Hg2+ fluorescence probe detection system.
The efficient management of food waste (FW) has emerged as a pivotal bottleneck impeding the sustainable development of the urban circular economy. This work employs a thermally activated persulfate (PDS) system to investigate abiotic carbon source production pathways of FW, aiming to achieve efficient resource utilization through biorefinery. The physicochemical properties, structure-activity relationship, and carbon source products analysis of FW during the conversion process revealed that the introduction of PDS significantly enhanced the hydrolysis and the dissolution of organic matter. Under the optimal conditions of PDS dosage of 0.2 mmol/g VS, 70 °C, and 1 h, the system achieved the highest carbon source production efficiency, with SCOD and TOC reaching 13726.0 ± 325.6 mg/L and 3862.0 ± 95.2 mg/L, respectively, representing increases of 35.2% and 26.6% relative to the control. The resulting FW-derived carbon source exhibited significantly elevated concentrations of volatile fatty acids, reducing sugars, and soluble sugars, indicating that complex particulate organic matter was effectively transformed into highly bioavailable low-molecular-weight compounds. EPR analysis elucidated that the ∙OH and ∙SO4- were the key reactive species driving the cleavage, depolymerization, and solubilization of macromolecular organic matter. Simultaneously, dissolved organic matter evolved from protein-like and microbially derived components toward humic-like substances, revealing the synergistic parallel characteristic of carbon source production and humification. In the batch nitrate utilization tests, the FW-derived carbon source achieved a denitrification rate of 70.56 mg/(g MLVSS·d), indicating its feasibility as an alternative external carbon source. As a technology for rapid carbon source production of FW, this approach is anticipated to enhance the utilization efficiency of low-grade resources, while facilitating waste resource utilization and offering feasible and important support for circular economy and sustainable urban development.
Grasslands are among the largest terrestrial biomes and play essential roles in livestock production, carbon sequestration and global food security. The productivity and resilience of these ecosystems are driven by complex molecular interactions between plants and their associated microbiomes. Although recent advances in nucleic acid research and multi-omics approaches have provided new insights into these interactions, the molecular mechanisms underpinning plant-microbiome interactions in these ecosystems remain insufficiently explored. This review synthesizes the latest progress in nucleic-acid and multi-omics approaches to better understand plant-microbiome interactions. It integrates nucleic acid-based technologies with multi-omics frameworks to explain plant-microbiome interactions across molecular, ecological, and management scales. By linking microbial community structure, functional genes, gene expression, metabolite profiles, ecosystem multifunctionality and sustainable grassland management, this review provides a broader framework for translating molecular insights into practical strategies for grassland resilience, productivity, and food security. Advances in amplicon sequencing, shotgun and long-read metagenomics, environmental DNA (eDNA) monitoring, plant and microbiome genome-wide association studies (GWAS) and transcriptomics have provided valuable insights into plant-microbiome interaction. This review highlights how these techniques enable functional and mechanistic understanding by linking microbial diversity with gene expression, nutrient cycling and plant performance. Additionally, long-read sequencing technologies provide genome-resolved analysis, improving the detection of structural and epigenetic variations, which are essential for understanding these interactions. These approaches reveal the role of beneficial microbes in enhancing grassland fertility, ultimately improving grassland productivity. Integrating these findings with metabolomics and phenomics offers a novel approach for predictive modeling in sustainable grassland management. The review concludes by emphasizing the need for standardized protocols, longitudinal field studies and experimental validation through synthetic communities and genome editing to harness plant-microbiome interactions for enhanced productivity and food security.
Cold stress (CS) is a major abiotic factor that significantly affects rice growth, development, and productivity. To withstand CS, rice plants have evolved intricate mechanisms that enable them to detect external signals and respond to changing environmental conditions. Understanding the physiological and molecular mechanisms underlying cold tolerance (CT) is therefore crucial for developing rice cultivars that can thrive under cold conditions. To date, a limited number of genes/QTLs associated with CT, such as: Ctb1, COLD1, COLD6, qLTG3-1, SGD1, and CTB4a, have been cloned and well studied. These genes play essential roles in the plant's response to CS by regulating various stress-related pathways, including reactive oxygen species (ROS) scavenging, osmotic regulation, and signal transduction. The physical co-localization of OsSRFP1 (associated with CT at the seedling stage) and OsMTACP2 (associated with CT at the reproductive stage) on chromosome 3 (between 13.1-13.3 Mb) suggests the potential for simultaneous introgression of these traits into recipient cultivars using donor parents carrying the desirable alleles. Genomic selection, powered by high-throughput genotyping technologies, enhances the accuracy and efficiency of identifying cold-tolerant genotypes. Additionally, integrating multi-omics approaches with gene-editing technologies offers a comprehensive strategy to uncover the complex molecular networks and regulatory pathways involved in CT, thereby identifying potential targets for genetic improvement. Overall, this review emphasizes the potential of integrating molecular, physiological, and genomic tools to develop rice cultivars resilient to CS, thereby contributing to global food security in the context of climate change. Cold stress severely limits rice growth and productivity, posing a significant threat to global food security. Despite some progress, cold tolerance remains a complex trait governed by intricate physiological and molecular mechanisms. This review offers comprehensive information on recent advances in understanding CT, highlighting how physiological responses are intertwined with molecular signalling pathways. By integrating traditional breeding with cutting-edge approaches such as GWAS, genomic selection, multi-omics, and gene editing, this work outlines a unified strategy to accelerate the development of cold-tolerant rice cultivars. This review provides valuable insights to enhance rice adaptation under climate variability and cold-prone environments.
Grains are staple foods for a large proportion of the global population and are characterized by high carbohydrate content, with proteins and lipids present in lower amounts. Among these macronutrients, specific bioactive lipids have been reported to exert physiological effects, including reducing blood pressure, decreasing the risk of cardiovascular and cerebrovascular diseases, and promoting intestinal health. Research on bioactive lipids has therefore attracted considerable attention. The intestinal tract is a major site for digestion and absorption and also serves as an important defense barrier against harmful external substances. Maintenance of intestinal homeostasis is closely associated with overall health. Studies have shown that certain active food components, including polysaccharides, polyphenols, and α-linolenic acid, regulate the physical, chemical, and immune barriers of the intestinal tract, as well as the gut microbiota. However, research on the effects of various bioactive ingredients in food on intestinal homeostasis in different physiological states remains incomplete, particularly regarding the regulatory effects of bioactive lipids. Further comprehensive studies are needed to clarify the mechanisms by which bioactive lipids regulate intestinal homeostasis. This review systematically summarizes the classification of bioactive lipids in grains, intestinal homeostasis, and current research on the regulatory effects of bioactive food ingredients, including bioactive lipids, on intestinal homeostasis, with the aim of providing a theoretical basis for future research. © 2026 Society of Chemical Industry.