Biofouling -- the occupation of microplastic (MP) surfaces by marine microbes -- alters particles' buoyancy and transport, yet its effect on the global distribution of MPs has not been well quantified. We present the first three-dimensional global Eulerian model to fully couple MP transport with biofouling, by augmenting the concentration field with an extra dimension representing the biomass attachment density on MP surfaces. This approach embeds time-dependent particle properties directly into the Eulerian concentration field, overcoming a fundamental challenge of tracking property evolution in grid-based models. Idealized simulations show that biofouling significantly reshapes the vertical distribution of MPs when two conditions are met: the particles must be sufficiently buoyant when they are clean to remain near the sea surface, and the local plankton growth rate must exceed the decay rate. In three-dimensional global simulations, biofouling substantially alters the distribution of large MPs ($\gtrsim 10$ $μ$m): biofouled particles are transported below the mixed layer to 500 m depth, and the subtropical surface garbage patches become more dispersed with reduced peak concentra
The escalating climate crisis and ecosystem degradation demand intelligent, low-cost sensors capable of robust, long-term monitoring in real-world environments. Absolute dissolved oxygen (DO) concentration is a key parameter for predicting climate tipping points. Inexpensive optoelectronic sensors based on microstructured polymer films doped with phosphorescent dyes could be readily deployable; however, signal drift and marine biofouling remain major challenges. Here, we introduce a sensing paradigm that combines camera-based DO sensors with a visual transformer (ViT)-based physics-informed neural network (PINN) for high-fidelity sensing under biofouling conditions. Training and testing data were obtained from an algae-laden water tank over 14 days to capture accelerated biofouling. The ViT-PINN, which embeds the Stern-Volmer (SV) equation into the loss function, reduces mean average error (MAE) by 92% and 89% compared to classical statistical and ML approaches, achieving ~2 umol/L absolute error. A deep ensemble further quantifies predictive uncertainty, enabling self-diagnostic sensing.
Marine biofouling on vessel hulls poses major ecological, economic, and biosecurity risks. Traditional survey methods rely on diver inspections, which are hazardous and limited in scalability. This work investigates automated classification of biofouling severity on the Level of Fouling (LoF) scale using both custom computer vision models and large multimodal language models (LLMs). Convolutional neural networks, transformer-based segmentation, and zero-shot LLMs were evaluated on an expert-labelled dataset from the New Zealand Ministry for Primary Industries. Computer vision models showed high accuracy at extreme LoF categories but struggled with intermediate levels due to dataset imbalance and image framing. LLMs, guided by structured prompts and retrieval, achieved competitive performance without training and provided interpretable outputs. The results demonstrate complementary strengths across approaches and suggest that hybrid methods integrating segmentation coverage with LLM reasoning offer a promising pathway toward scalable and interpretable biofouling assessment.
STRings for Absorption Length in Water (STRAW)-a and b were pathfinder instruments deployed to characterize the anticipated site of the Pacific Ocean Neutrino Experiment (P-ONE), which is a future neutrino telescope that will be located in the North Pacific Ocean. Measurements of the evolution of the optical transmission efficiency from STRAW-a showed a decline over the detector's lifetime for the upward-facing modules. Video footage of the pathfinders strongly suggested this decline was caused by biofouling and sedimentation. We measure the effect of biofouling and sedimentation to be a decrease in the transparency of upward-facing optical surfaces over 5 years of operations. A majority of downward-facing optical surfaces, which will dominate P-ONE's sensitivity to astrophysical sources, showed no visible biofouling. Extrapolations motivated by biological growth models estimated that these losses started around 2.5 years after deployment, and suggest a reduction in transparency ranging from 35$\%$ of the original to complete obscuration for the upward-facing modules. Samples of biofouling were taken in order to identify the microbial diversity of these organisms and inform potenti
Biofouling$\unicode{x2013}$communities of organisms that grow on hard surfaces immersed in water$\unicode{x2013}$provides a pathway for the spread of invasive marine species and diseases. To address this risk, international vessels are increasingly being obligated to provide evidence of their biofouling management practices. Verification that these activities are effective requires underwater inspections, using divers or underwater remotely operated vehicles (ROVs), and the collection and analysis of large amounts of imagery and footage. Automated assessment using computer vision techniques can significantly streamline this process, and this work shows how this challenge can be addressed efficiently and effectively using the interpretable Component Features (ComFe) approach with a DINOv2 Vision Transformer (ViT) foundation model. ComFe is able to obtain improved performance in comparison to previous non-interpretable Convolutional Neural Network (CNN) methods, with significantly fewer weights and greater transparency$\unicode{x2013}$through identifying which regions of the image contribute to the classification, and which images in the training data lead to that conclusion. All cod
The process of fish cage inspections, which is a necessary maintenance task at any fish farm, be it small scale or industrial, is a task that has the potential to be fully automated. Replacing trained divers who perform regular inspections with autonomous marine vehicles would lower the costs of manpower and remove the risks associated with humans performing underwater inspections. Achieving such a level of autonomy implies developing an image processing algorithm that is capable of estimating the state of biofouling buildup. The aim of this work is to propose a complete solution for automating the said inspection process; from developing an autonomous control algorithm for an ROV, to automatically segmenting images of fish cages, and accurately estimating the state of biofouling. The first part is achieved by modifying a commercially available ROV with an acoustic SBL positioning system and developing a closed-loop control system. The second part is realized by implementing a proposed biofouling estimation framework, which relies on AI to perform image segmentation, and by processing images using established computer vision methods to obtain a rough estimate of the distance of the
In recent years, there has been an increasing demand for underwater cameras that monitor the condition of offshore structures and check the number of individuals in aqua culture environments with long-period observation. One of the significant issues with this observation is that biofouling sticks to the aperture and lens densely and prevents cameras from capturing clear images. This study examines an underwater camera that applies material technologies with high inherent resistance to biofouling and computer vision technologies based on image reconstruction by deep learning to lens-less cameras. For this purpose, our prototype camera uses a coded aperture with 1k rectangular shape pinholes in a thin metal plate, such as copper, which hinder the growth of biofouling and keep the surface clean. Although images taken by lens-less cameras are usually not well formed due to lack of the traditional glass-based lens, a deep learning approach using ViT (Vision Transformer) has recently demonstrated reconstructing original photo images well and our study shows that using gated MLP (Multilayer Perceptron) also yields good results. On the other hand, a certain degree of thickness for bio-rep
Biofouling represents a critical challenge in marine transportation, healthcare, and food manufacturing, among other industries, as it promotes contamination and increases maintenance costs. Zwitterionic polymers, known for their exceptional antifouling properties, offer a promising solution for biofouling deterrence. Despite the rapid development of zwitterionic polymers in recent years, the design rules, especially concerning the choice of cationic moieties to optimize biofouling deterrence, remain elusive. In this study, we leveraged a versatile all-dry synthesis scheme to achieve a selection of 9 zwitterionic polymers, 5 of which are unprecedented for this synthesis paradigm, thus systematically unraveling that molecular design rule. Notably, we developed a synthesis strategy to enable nanoscale compositional gradient along the coating cross-section, which ensures the robustness of the zwitterionic polymer coatings irrespective of the choice of cation-anion combinations. That robustness is enabled by an organosilicon-based layer at the coating-substrate interface, which simultaneously enhances coating adhesion and chemical stability while ensuring high concentration of zwitteri
Biofouling of ships causes major environmental and economic consequences all over the world. In addition, biofouling management of ship hulls causes both social, environmental and economic risks that should all be considered reaching well-balanced decisions. In addition, each case is unique and thus optimal management strategy must be considered case-specifically. We produced a novel decision support tool using Bayesian networks to promote the comprehensive understanding about the complex biofouling management issue in the Baltic Sea and to identify potential management options and their consequences. The tool compares the biofouling management strategies in relation to NIS (non-indigenous species) introduction risk, eco-toxicological risk due to biocidal coating, carbon dioxide emissions resulting from fuel consumption and costs related to fuel consumption, in-water cleaning and coating. According to the results, the optimal biofouling management strategy would consist of a biocidal-free coating with regular in-water cleaning and with devices collecting the material. However, the best biocidal-free coating type and the optimal in-water cleaning interval varies and depends e.g. on
Water monitoring is crucial for environmental monitoring, transportation, energy and telecommunication. One of the main problems in aquatic environmental monitoring is biofouling. The simplest method among the current antifouling strategies is the use of wiper technologies like brushes and wipers which apply mechanical pressure. In designing built-in strategies however, manufacturers usually build the sensor around the biofouling system. The current state-of-the-art is a fully integrated central wiper in the sensor that enables cleaning of all probes mounted on the sonde. Improvements in antifouling strategies lag rapid advancements in sensor technologies such as in miniaturization, specialization, and costs. Hence, improving built-in designs by decreasing size and complexity will decrease maintenance and overall costs. This design is targeted for the EU project Robocoenosis since bio-hybrid systems in this project incorporate living organisms. This technology targets selective proliferation of the organisms which only prevents biofilms on components where they are unwanted. Beyond this, the use of autonomous activation based on image processing may likely be advantageous for minim
Biofouling is the accumulation of organisms on surfaces immersed in water. It is of particular concern to the international shipping industry because it increases fuel costs and presents a biosecurity risk by providing a pathway for non-indigenous marine species to establish in new areas. There is growing interest within jurisdictions to strengthen biofouling risk-management regulations, but it is expensive to conduct in-water inspections and assess the collected data to determine the biofouling state of vessel hulls. Machine learning is well suited to tackle the latter challenge, and here we apply deep learning to automate the classification of images from in-water inspections to identify the presence and severity of fouling. We combined several datasets to obtain over 10,000 images collected from in-water surveys which were annotated by a group biofouling experts. We compared the annotations from three experts on a 120-sample subset of these images, and found that they showed 89% agreement (95% CI: 87-92%). Subsequent labelling of the whole dataset by one of these experts achieved similar levels of agreement with this group of experts, which we defined as performing at most 5% wo
Thin-film self-assembly of three-dimensional (3D) microsystems presents a compelling route to integrate complex functionalities into ultra-compact volumes, yet strategies for incorporating tunable ion-conducting elements remain limited. Here, we introduce a strain-induced self assembly platform that transforms lithographically patterned multilayer thin films into functional 3D coaxial Swiss-roll microtubes with total active volumes below 1 uL. A key innovation is the monolithic integration of a chemically tunable polyimide proton-exchange membrane, enabling post-fabrication optimization of ionic transport that balances proton transport with mediator blocking. We further implement a dual-mode operational scheme that decouples microbial metabolism from electrochemical power generation, revealing biofouling, not chemical fouling or membrane degradation, as the dominant failure mechanism in conventional architectures. Critically, optimally treated polyimide membranes exhibit excellent recoverability after fouling, while cell-free mode operation maintains stable performance by physically excluding microorganisms from the microelectronic environment. This integrated bio-electronic micros
The movement of microorganisms near solid-liquid interfaces is a topic of significant scientific interest due to its relevance in various natural and industrial contexts, such as biofilm formation and marine biofouling. In this study, we investigate the swimming behavior of C. reinhardtii near a sinusoidal periodic microstructure (SPM). Using fluorescence microscopy and three-dimensional tracking, we observe that the swimming direction of C. reinhardtii is strongly influenced by the geometric constraints of the SPM. Our results show that cells tend to aggregate at the bottom of the SPM rather than the top, and exhibit a speed orientation tendency near the microstructure. We attribute this behavior to a combination of the "memory effect" and hydrodynamic attraction. By altering the shape of the periodic microstructure, we successfully achieve directed induction of cell swimming, which has potential applications in micro-nano robot control and biofouling prevention. This work provides new insights into the movement mechanisms of microorganisms near solid-liquid surfaces and highlights the potential for manipulating their behavior through microstructure design.
A moisture-driven air capture system was developed and demonstrated for cultivating cyanobacteria and microalgae at the flask (50 mL), bench (12 L) and small pilot (840 L) scale. Purolite A501 anion exchange resin beads were found to be biocompatible and rapidly deliver air-captured CO2 when immersed directly in an alkaline cultivation medium containing cyanobacteria or microalgae. Flask-scale cultivation trials showed A501 could sustain rapid growth (190 mg/L/d) of the cyanobacterium Synechocystis sp. PCC 6803 strain engineered to produce laurate. A bench-scale system installed in a laminar flow hood was able to deliver 2 g CO2/d into abiotic alkaline cultivation medium and 0.5 g/d in the presence of Synechocystis to support vigorous growth (39 mg/L/d) limited by the CO2 delivered by the sorbent. A small pilot-scale system installed in a 4.2 m2 outdoor raceway pond in Mesa, Arizona was able to deliver 100 g CO2/d into abiotic alkaline cultivation medium. Exopolysaccharides and other products excreted by Synechocystis 6803 covered the sorbent beads, reducing their capacity to 25%, which could be partially restored to 70% capacity using a wash protocol, but the CO2 delivery kinetics
Effective management of cooling tower systems requires thorough disinfection. While traditional chemical water treatment methods are currently the most prominent strategy, they are costly and yield limited results when relied upon as the sole approach. Cross-flow microsand filtration systems offer a promising alternative with the added benefit of potentially increasing evaporative cooling efficiency, thus saving energy. A comprehensive data-driven analysis over two cooling seasons evaluated the energetic performance of a system equipped with and without an operating filter. For similar environmental conditions, the coefficient of performance was on average 18% higher and was higher 63% of the time when the filter was operating, indicating superior heat transfer efficiency and significant energy savings. It was also 41% higher during periods of high cooling demand. Consequently, the filter and the system work more efficiently at high wet-bulb temperature and thermal load. Machine learning modeling suggested that operating the filter year-round could save between 5% and 13% of the energy bill, primarily during the cooling season. Continuous filter operation is essential as it mitigat
Aquaculture is a thriving food-producing sector producing over half of the global fish consumption. However, these aquafarms pose significant challenges such as biofouling, vegetation, and holes within their net pens and have a profound effect on the efficiency and sustainability of fish production. Currently, divers and/or remotely operated vehicles are deployed for inspecting and maintaining aquafarms; this approach is expensive and requires highly skilled human operators. This work aims to develop a robotic-based automatic net defect detection system for aquaculture net pens oriented to on- ROV processing and real-time detection of different aqua-net defects such as biofouling, vegetation, net holes, and plastic. The proposed system integrates both deep learning-based methods for aqua-net defect detection and feedback control law for the vehicle movement around the aqua-net to obtain a clear sequence of net images and inspect the status of the net via performing the inspection tasks. This work contributes to the area of aquaculture inspection, marine robotics, and deep learning aiming to reduce cost, improve quality, and ease of operation.
Microfluidic channels with embedded ion permselective medium under the application of electric current are commonly used for electrokinetic processes as on-chip ion concentration polarization (ICP) and bioparticle preconcentration to enhance biosensing. Herein, we demonstrate the ability to dynamically control the electrically driven ion transport by integrating individually addressable microvalves. The microvalves are located along a main microchannel that is uniformly coated with a thin layer of an ion-exchange membrane (IEM). The interplay of ionic transport between the solution within the microchannel and the thin IEM, under an applied electric current, can be locally tuned by the deformation of the microvalve. This tunability provides a robust and simple means of implementing new functionalities into lab-on-a-chip devices, e.g., dynamic control over multiple ICP layers and their associated preconcentrated molecule plugs, multiplex sensing, suppression of biofouling as well as plug dispersion, while maintaining the well-known application of microvalves as steric filtration.
Biofouling is a major challenge for sustainable shipping, filter membranes, heat exchangers, and medical devices. The development of fouling-resistant coatings requires the evaluation of their effectiveness. Such an evaluation is usually based on the assessment of fouling progression after different exposure times to the target medium (e.g., salt water). The manual assessment of macrofouling requires expert knowledge about local fouling communities due to high variances in phenotypical appearance, has single-image sampling inaccuracies for certain species, and lacks spatial information. Here we present an approach for automatic image-based macrofouling analysis. We created a dataset with dense labels prepared from field panel images and propose a convolutional network (adapted U-Net) for the semantic segmentation of different macrofouling classes. The establishment of macrofouling localization allows for the generation of a successional model which enables the determination of direct surface attachment and in-depth epibiotic studies.
We study the behavior of hydrated graphite oxide (GO) at high temperatures using thermally accelerated molecular dynamics simulations based on ab initio density functional theory. Our results suggest that GO, a viable candidate for water treatment and desalination membranes, is more heat resilient than currently used organic materials. The system we consider to represent important aspects of thermal processes in highly disordered GO is a hydrated GO bilayer in vacuum. Our study provides microscopic insight into reactions involving water and functional epoxy-O and OH-groups bonded to graphene layers, and also describes the swelling of the structure by water vapor pressure at elevated temperatures. We find the system to withstand simulation temperatures up to ${\approx}$2,500 K before the graphitic layers start decomposing, implying the possibility of cleaning biofouling residue from a GO-based membrane by heating in an inert gas atmosphere.
The chemical composition and structural state of advanced alloys are the decisive factors in optimum biomedical performance. This contribution presents unique Ti-Zr-Ge metallic glass thin-film compositions fabricated by magnetron sputter deposition targeted for nanocoatings for biofouling prevention. The amorphous nanofilms with nanoscale roughness exhibit a large relaxation and supercooled liquid regions as revealed by flash differential scanning calorimetry. Ti\textsubscript{68}Zr\textsubscript{8}Ge\textsubscript{24} shows the lowest corrosion (0.17 \textmu A cm\textsuperscript{\textminus2}) and passivation (1.22 \textmu A cm\textsuperscript{\textminus2}) current densities, with the lowest corrosion potential of \textminus0.648 V and long-range stability against pitting, corroborating its excellent performance in phosphate buffer solution at 37 {\textdegree}C. The oxide layer is comprised of TiO\textsubscript{2}, TiO\textsubscript{\emph{x}} and ZrO\textsubscript{\emph{x}}, as determined using X-ray photoelectron spectroscopy by short-term ion-etching of the surface layer. The two orders of magnitude increase in the oxide and interface resistance (from 14 to 1257 {\textOmega} cm\t