[Preparation of B@PH/GM-V hydrogel microspheres and their antioxidant and osteogenesis-promoting effects].

内容摘要

This study constructed bone morphogenetic protein 2-loaded polydopamine heparin nanoparticles (B@PH NPs) and vanillin methacrylate (VMA)-grafted GelMA hydrogel microspheres (B@PH/GM-V) and evaluated their physicochemical properties and regulatory effects on osteogenic differentiation under oxidative stress. B@PH NPs were prepared through oxidative self-polymerization, and B@PH/GM-V microspheres were fabricated via microfluidic technology. The materials were characterized by using scanning electron microscope, Fourier transform infrared spectrometer (FTIR), and energy-dispersive X-ray spectroscopy (EDS). Swelling, degradation, and drug release behaviors were evaluated. Biocompatibility was assessed through the CCK-8 assay and live/dead staining by using MC3T3-E1 cells. Intracellular reactive oxygen species (ROS) levels were detected with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) probes, and osteogenic differentiation capacity was evaluated through alkaline phosphatase and alizarin red S staining. The average diameters of B@PH NPs and B@PH/GM-V microspheres were approximately 288.2 nm and 499.1 μm, respectively. FTIR and EDS confirmed successful modification with heparin and VMA. B@PH NPs showed favorable sustained release performance. B@PH/GM-V exhibited good biocompatibility, significantly reduced the ROS levels induced by lipopolysaccharide and H2O2, and promoted the differentiation and mineralization of MC3T3-E1 cells. B@PH/GM-V hydrogel microspheres possess good biocompatibility, antioxidant activity, and osteogenic effects, showing promising potential for periodontitis-related alveolar bone regeneration. 目的: 构建负载骨形态发生蛋白-2的聚多巴胺肝素纳米颗粒（B@PH NPs），并与接枝甲基丙烯酸香兰素（VMA）的GelMA复合，制备B@PH/GM-V水凝胶微球，评价其理化性能及在氧化应激微环境中对成骨分化的调控作用。方法: 采用氧化自聚合法制备B@PH NPs，微流控法制备B@PH/GM-V水凝胶微球;通过扫描电镜、傅里叶变换红外光谱（FTIR）、能谱分析（EDS）进行材料表征;检测溶胀、降解及体外释药行为。以MC3T3-E1细胞为模型，采用CCK-8法、活/死细胞染色评价生物相容性;2'，7'‑二氯二氢荧光素二乙酸酯（DCFH-DA）荧光探针检测细胞内活性氧（ROS）水平;碱性磷酸酶与茜素红S染色评估成骨分化能力。结果: B@PH NPs平均粒径为288.2 nm，B@PH/GM-V微球平均直径为499.1 μm。FTIR与EDS结果证实肝素与VMA成功修饰。B@PH NPs具有良好缓释效果。细胞实验显示，B@PH/GM-V生物相容性优良，可显著降低脂多糖和H2O2诱导的ROS水平，并促进MC3T3-E1细胞分化与矿化。结论: B@PH/GM-V水凝胶微球具有良好生物相容性、抗氧化能力与促成骨效应，在牙周炎相关牙槽骨再生修复中具有潜在应用价值。.