Enhancing feed value of broom sorghum stover silage via Lactiplantibacillus plantarum, cellulase, and xylanase: a comprehensive analysis of fermentation quality, microbiota, volatile organic compounds, and anti-nutrients.

内容摘要

This study Aimed to evaluate the effects of Lactiplantibacillus plantarum (LP), cellulase (CE) or xylanase (XE) on the fermentation quality and microbial community of the broom sorghum stover silage. It was found that additives can improve the fermentation quality and digestibility of broom sorghum stover. Compared with the CK treatment, the addition of cellulase significantly increased the CP and WSC contents of broom sorghum stover, and also significantly improved the in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility. The microbial community in broom sorghum stover silage also changed with different additive treatments. The microbiota shifted from Pantoea and Nissabacter to Lactiplantibacillus and Lentilactobacillus. Changes in microorganisms indicate a decrease in the number of harmful microorganisms and more retention of nutrients. All treatments reduced pungent VOCs (e.g., Benzyl isothiocyanate) and enriched aromatic VOCs (e.g., Phenol 4-ethyl-, β-damascenone), with the CE treatment performing best. Ensiling degraded anti-nutritional factors: the CK and LP treatments were superior for tannin reduction, the CE and XE treatments for phytic acid. These additives effectively enhance broom sorghum stover's feed value, with CE showing the most comprehensive effect, providing a basis for its high-value utilization and sustainable livestock production.