Effects of a 14-strain Probiotics Supplement on Salivary Antimicrobial Proteins at Rest and in Response to an Acute Bout of Prolonged Exercise

内容摘要

This study was designed to determine the effects of a period of supplementation with a 14-strain probiotics supplement on salivary antimicrobial proteins (AMPs) at rest and in response to an acute bout of prolonged exercise in active, healthy adults. In this study, 11 participants (age: 22 ± 1 years, weight: 69.5 ± 12.2 kg, body mass index: 23.0 ± 1.8 kg/m 2 ) cycled for 2 h at 60% VO2max (maximum oxygen uptake) on 2 occasions (2 trials separated by 30 days). During each trial, participants came to the lab in the morning, 2 h after having their breakfast. They were asked to have a similar breakfast before each trial. The second trial was performed after 30 days of supplementation with a 14-strain probiotics. The probiotics were in capsule form and taken as 1 capsule 3 times daily (morning, midday, and evening). Timed, unstimulated saliva samples were collected pre-, post-, 1 h post-, and 2 h post-exercise. Saliva samples were analysed for secretory immunoglobulin A (S-IgA), alpha (α)-amylase, lactoferrin, and lysozyme concentrations. Results showed that 30 days of supplementation with the 14-strain probiotics did not alter salivary AMPs at rest and in response to an acute bout of prolonged exercise. However, prolonged exercise significantly increased lactoferrin concentration and α-amylase activity post-exercise. It was concluded that the supplementation period and/or the dose/concentration of the 14-strain probiotics used in this study were insufficient to induce any beneficial effects on athletes' salivary AMPs at rest and in response to an acute bout of prolonged exercise.